E-Cadherin is a classical member of the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats; a transmembrane region; and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric; breast; colorectal; thyroid; and ovarian cancers. Loss of function is thought to contribute to progression in cancer by increasing proliferation; invasion; and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells; and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Also; E-Cadherin has a potent invasive suppressor role and it is a ligand for integrin alpha-E/beta-7.
Product Name:
Human E-Cadherin/CDH1 Recombinant Protein (RPES4314)
Recombinant Human Cadherin-1/E-Cadherin/CDH1 is produced by our Mammalian expression system and the target gene encoding Asp155-Ile707 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
61.2 kDa
AP Mol Mass:
80-90 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
