Immunoglobulin G (IgG) is a monomeric immunoglobulin that plays an important part in the secondary antibody response and is the only isotype that may pass through the human placenta. Immunoglobulins are composed of 75% of serum immunoglobulins in humans and are produced by plasma B cells. Antibodies from the IgG class protect against bacteria by agglutination and immobilization, complement activation, toxin neutralization, and antibody-dependent cell-mediated cytotoxicity (ADCC). The IgG tetramer has two heavy chains (50 kDa) and two light chains (25kDa), linked by disulfide bonds, which form a Y-like structure. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is the most prevalent in serum among the four IgG subcategories (IgG1, 2, 3, and 4) and binds with high affinity to Fc receptors on phagocytic cells. Fc fragment is demonstrated to mediate phagocytosis; trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal bacteria, which binds tightly with the Fc region of IgGs, has many applications in biotechnology as a reagent for affinity purification. The recombinant IgG Fc region is considered to be a potential anti-inflammatory medication for the treatment of human autoimmune diseases. Human IgG2-Fc Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IgG2-Fc Recombinant Protein (RPES2197)
Product Code:
RPES2197
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Ig gamma-2 chain C region,IgG2 Fc
Accession:
P01859
Sequence:
Val104-Lys326(Val161Met)
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human IgG2-Fc is produced by our Mammalian expression system and the target gene encoding Val104-Lys326(Val161Met) is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
25.1 kDa
AP Mol Mass:
32 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
