Description
SuperSet DIY Accessory Pack
Complementary materials and reagents needed for use with SuperSet ELISA development kits from ELISA Genie. Reagents are provided to be sufficient for 5 plates.
| Material / Reagent | Quantity | Description |
|---|---|---|
| 96 wells microtiter plate | 5 | 12 removable strips |
| Plastic plate covers | 10 | Adhesive sealers. |
| Cover the plate during assay incubations | ||
| Plate storage material | 5 | Plastic bag and desiccant to store plate once coated |
| (up to 12 months at 2-8°C) | ||
| Reconstitution Buffer (0.1ml) | 1 | 10X concentrate. |
| Add 0.9 ml of distilled water directly in the vial | ||
| See section 9.3 of the ELISA SuperSet Insert | ||
| Coating Buffer (5ml) | 1 | 10X concentrate. Sterile |
| For one plate, add 1 ml to 9 ml of distilled water See section 8.1 of the ELISA SuperSet Insert | ||
| Wash Buffer (25ml) | 1 | 200X concentrate |
| For one plate, add 5 ml to 995 ml of distilled water | ||
| Blocking Buffer (10ml) | 5 | Ready to use. Sterile |
| See section 8.2 of the ELISA SuperSet Insert | ||
| To be discarded and not washed after the addition | ||
| Standard Dilution Buffer (12.5ml) | 1 | 10X concentrate |
| For one plate, add 2.5 ml to 22.5 ml of distilled water See section 9.2 of the ELISA SuperSet Insert | ||
| To be used for the dilution of samples if necessary | ||
| Secondary Antibody Dilution Buffer (25ml) | 1 | Ready to use |
| See section 9.3 of the ELISA SuperSet Insert | ||
| HRP Dilution Buffer (25ml) | 2 | Ready to use |
| See section 9.4 of the ELISA SuperSet Insert | ||
| Stop Reagent (10ml) | 5 | Ready to use 1N Sulfuric Acid Solution |
Storage Instructions:
- Store unopened accessory pack at 2-8°C.
- All reagents should be warmed to room temperature before use. If crystals have formed in concentrated Buffer (Wash and Standard Dilution): warm it gently until they have completely dissolved.
- Immediately after use remaining reagents and should be returned to cold storage (2-8°C).
- Once prepared, store diluted buffers 1X at 2-8°C for up to one week.
- Expiry of the reagents is stated on box front labels.
- Do not use components past the expiration date.
Safety & Precautions
- The Stop Solution provided is an acid solution.
- Reconstitution, Standard Dilution, Secondary Antibody Dilution and HRP Dilution Buffers contain ProClin which may cause an allergic skin reaction.
- Wear appropriate clothing, gloves and eye protection.
