Description
COVID-19 Human IgG PharmaGenie ELISA Kit - Kit Data
| Product SKU: | SBRS1729 |
| Size: | 96T |
| Gene Names: | IGG |
| Target Species: | Human |
| Compatible Sample Types: | Dried Blood, Plasma, Saliva, Serum |
| Design Principle: | Indirect |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C. |
Kit Components:
| 1. | SARS-CoV-2 N-protein coated 96-well microplate |
| 2. | Wash Buffer |
| 3. | Positive Control |
| 4. | Biotinylated Anti-Human IgG |
| 5. | HRP-Streptavidin concentrate |
| 6. | TMB One-Step Substrate |
| 7. | Stop Solution |
| 8. | Assay Diluent |
| 9. | 5X Sample Diluent |
| 10. | 1X PBS (DBS version) |
| 11. | Tween-20 (DBS version) |
| 12. | Protease Inhibitor Cocktail (DBS version) |
Other materials and equipment required:
The Assay Genie COVID-19 Human IgG ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Distilled or deionized water
Kit Protocol:
| 1. | Prepare all reagents, samples and standards as instructed. |
| 2. | Add 100 ul positive control or sample to each well. Incubate 1 hour at room temperature. |
| 3. | Add 100 µl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature. |
| 4. | Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature. |
| 5. | Add 100 ul TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature. |
| 6. | Add 50 ul Stop Solution to each well. Read at 450 nm immediately. |
