Fumarase Activity Assay Kit
FUMARASE (or fumarate hydratase) (EC 188.8.131.52) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.Assay Genie's non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
ApplicationsFor quantitative determination of fumarase enzyme activity.
- Fast and sensitive. Linear detection range: 0.4 to 70 U/L for 30 min reaction at 37°C.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Fumarase Activity Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL Enzyme A: 120 uL NAD/MTT: 1 mL Enzyme B: 120 uL Substrate: 600 uL Calibrator: 1.5 mL|
|Kit Requires:||Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader|
|Method of Detection:||OD565 nm|
|Detection Limit:||0.4 U/L|
|Samples:||Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)|
|Protocol Length:||40 min|
|Storage:||Store all components at -20°C upon receiving.|
|Shelf Life:||6 months|