The Human SNCgamma (Synuclein Gamma) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of SNCgamma levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a wide array of research applications.SNCgamma, also known as Gamma-Synuclein, plays a key role in various cellular processes, including synaptic function and neurodegeneration. Dysregulation of SNCgamma has been implicated in neurodegenerative disorders such as Parkinson's disease, making it a critical biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human SNCgamma CLIA Kit, researchers can gain valuable insights into the role of SNCgamma in health and disease, paving the way for new discoveries and advancements in the field of neurobiology. Trust Assay Genie to provide you with the tools you need for groundbreaking research.
Product Name:
Human SNCgamma (Synuclein Gamma) CLIA Kit
SKU:
HUES00804
Target:
Human SNCgamma (Synuclein Gamma)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SNCγ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SNCγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SNCγ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SNCγ. You can calculate the concentration of Human SNCγ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
91-103
85-97
Average (%)
110
97
90
1:4
Range (%)
83-95
98-111
91-105
Average (%)
90
104
97
1:8
Range (%)
85-101
102-119
92-104
Average (%)
92
109
97
1:16
Range (%)
102-119
96-111
88-101
Average (%)
108
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
98-109
103
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.14
444.38
1752.85
221.16
464.6
1735.84
Standard deviation
18.92
44.13
188.26
28.31
54.5
191.64
C V (%)
8.96
9.93
10.74
12.8
11.73
11.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SNCγ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SNCγ in samples. No significant cross-reactivity or interference between Human SNCγ and analogues was observed.
