Description
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Mouse Cytokine Array (144 targets) (SARB0079)
The Mouse Cytokine Array - 144 Targets (SARB0079) is a powerful tool for researchers studying cytokine signaling in various biological samples. This array allows for the simultaneous detection of 144 different mouse cytokines, providing valuable insights into the complex network of signaling molecules involved in immune response, inflammation, and other biological processes.With high sensitivity and specificity, the Mouse Cytokine Array - 144 Targets (SARB0079) enables the quantification of cytokine levels in a rapid and efficient manner. This array is suitable for a wide range of sample types, including serum, plasma, cell culture supernatants, and tissue lysates, making it highly versatile for researchers in diverse fields such as immunology, cancer biology, and infectious diseases.
By using this cytokine array, researchers can gain a comprehensive view of cytokine profiles in their samples, leading to a better understanding of cytokine-mediated immune responses and disease pathogenesis. This valuable tool can help accelerate research efforts aimed at developing novel therapies and interventions targeting cytokine signaling pathways.
| Product Sku: | SARB0079 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 144 |
| Gene Symbols: | ACE, AGER, AREG, AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2|CCL20, CCL21, CCL22, CCL24, CCL25, CCL27, CCL3, CCL5, CCL9, CD27, CD36, CD40, CD40LG, CD70, CDH1, CHRD, CSF1, CSF2, CSF2RB, CSF3, CTF1, CTLA4, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL15, CXCL16, CXCL2, CXCL5, CXCL9, DCN, DKK1, DPP4, EGF, ENG, EPGN, EREG, F11R, FASLG, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4, GAS1, GAS6, GZMB, HGF, ICAM1, IFNG, IFNL2, IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL10, IL11, IL12A, IL13, IL15, IL17A, IL17B, IL17F, IL17RB, IL1A, IL1B, IL1RL1, IL1RN, IL2, IL20, IL21, IL25, IL2RA, IL3, IL4, IL5, IL6, IL6R, IL7, IL9, KDR, KITLG, LEP, LEPR, LGALS1, MADCAM1, MET, MFGE8, MME, MMP2, MMP3, MMP9, PF4, PPBP, PRL, PTX3, RETN, SELE, SELL, SELP, SHH, SLPI, SPINT1, SPP1, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF13B, TNFRSF18, TNFRSF19, TNFRSF8, TNFRSF9, TNFSF11, TNFSF12, TNFSF18, TNFSF8, TNFSF9, TPO, TREM1, TSLP, TYRO3, VCAM1, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| 6Ckine (CCL21)
| ACE
| ALK-1
| Amphiregulin
|
Axl
| bFGF
| BLC (CXCL13)
| Cardiotrophin-1 (CT-1)
| CD27 (TNFRSF7)
|
CD27 Ligand (TNFSF7)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
| CD36 (SR-B3)
| CD40 (TNFRSF5)
|
CD40 Ligand (TNFSF5)
| Chordin
| CRG-2
| CTACK (CCL27)
| CTLA-4 (CD152)
|
CXCL16
| Decorin
| DKK-1
| CD26 (DPPIV)
| Dtk
|
E-Cadherin
| EGF
| Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Epigen
| Epiregulin
| E-Selectin
| Fas Ligand (TNFSF6)
| Fc gamma RIIB (CD32b)
|
Flt-3 Ligand
| Fractalkine (CX3CL1)
| Galectin-1
| GCSF
| GITR (TNFRSF18)
|
GITR Ligand (TNFSF18)
| GM-CSF
| Granzyme B
| Gas 1
| Gas 6
|
HAI-1
| HGF
| HGFR
| ICAM-1 (CD54)
| IFN-gamma
|
IGFBP-2
| IGFBP-3
| IGFBP-5
| IGFBP-6
| IGF-1
|
IGF-2
| IL-1 beta (IL-1 F2)
| IL-1 R4 (ST2)
| IL-10
| IL-11
|
IL-12 p40/p70
| IL-12 p70
| IL-13
| IL-15
| IL-17A
|
IL-17B
| IL-17 RB
| IL-17E (IL-25)
| IL-17F
| IL-1 alpha (IL-1 F1)
|
IL-1 RA (IL-1 F3)
| IL-2
| IL-2 R alpha
| IL-20
| IL-21
|
IL-28A (IFN-lambda 2)
| IL-3
| IL-3 R beta
| IL-4
| IL-5
|
IL-6
| IL-6 R
| IL-7
| IL-9
| I-TAC (CXCL11)
|
JAM-A (CD321/F11R)
| KC (CXCL1)
| Leptin
| Leptin R
| LIX
|
L-Selectin (CD62L)
| Lungkine (CXCL15)
| Lymphotactin (XCL1)
| MAdCAM-1
| MCP-1 (CCL2)
|
MCP-5
| M-CSF
| MDC (CCL22)
| MFG-E8
| MIG (CXCL9)
|
MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
|
MMP-2
| MMP-3
| Neprilysin
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
|
Pentraxin-3 (TSG-14)
| Platelet Factor 4 (CXCL4)
| Prolactin
| Pro-MMP-9
| P-Selectin
|
RAGE
| RANTES (CCL5)
| Resistin
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
Sonic Hedgehog N-Terminal (Shh-N)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TACI (TNFRSF13B)
| TARC (CCL17)
|
I-309 (TCA-3/CCL1)
| TECK (CCL25)
| TCK-1 (CXCL7)
| TIMP-1
| TIMP-2
|
TNF alpha
| Thrombopoietin (TPO)
| TRANCE (TNFSF11)
| TREM-1
| TROY (TNFRSF19)
|
TSLP
| TWEAK (TNFSF12)
| TWEAK R (TNFRSF12)
| VCAM-1 (CD106)
| VEGF-A
|
VEGFR1
| VEGFR2
| VEGFR3
| VEGF-D
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C2000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
