The [KO Validated] PARP1 Antibody (CAB0942) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blotting applications.PARP1 plays a significant role in DNA damage response and is a potential target for cancer therapy. By binding to PARP1, this antibody enables the detection and analysis of PARP1 expression levels in different cell types, facilitating research in fields such as oncology, genomics, and drug development.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] PARP1 Antibody
SKU:
CAB0942
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
HeLa treated by staurosporine, Jurkat, A-549, Mouse testis, Rat testis
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
113kDa
Observed MW:
89kDa/113kDa
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Purification Method
Affinity purification
Gene ID
142
RRID
AB_2757470
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and PARP1 knockout (KO) HeLa cells, using [KO Validated] PARP1 Rabbit pAb (CAB0942) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
