The RIPK1/RIP Monoclonal Antibody (CAB19580) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. RRID AB_2862680 Gene ID 8737 Swiss Prot Synonym RIP; RIP1; AIEFL; IMD57; RIP-1; RIPK1/RIP
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RIPK1/RIP Monoclonal Antibody
SKU:
CAB19580
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0059
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RIP, RIP1, AIEFL, IMD57, RIP-1, RIPK1/RIP
Positive Sample:
Raji, HeLa, 293T
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
76kDa
Observed MW:
76kDa
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. RRID AB_2862680 Gene ID 8737 Swiss Prot Synonym RIP; RIP1; AIEFL; IMD57; RIP-1; RIPK1/RIP
Purification Method:
Affinity purification
Gene ID:
8737
RRID:
AB_2862680
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KD Validated] RIPK1/RIP Rabbit mAb (CAB19580) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of lysates from wild type (WT) and RIPK1/RIP knockdown (KD) 293T cells using [KD Validated] RIPK1/RIP Rabbit mAb (CAB19580) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
