The C1orf146 Antibody (CAB18804) is a high-quality antibody developed for reliable detection and analysis of target proteins. Involved in reciprocal meiotic recombination and synaptonemal complex assembly. Located in chromosome. Is expressed in ovary; spermatid; spermatocyte; spermatogonium; and testis. Orthologous to human C1orf146 (chromosome 1 open reading frame 146).
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
C1orf146 Antibody
SKU:
CAB18804
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SCRE, Spo16, C1orf146
Positive Sample:
Rat testis
Calculated MW:
21kDa
Observed MW:
20kDa
Involved in reciprocal meiotic recombination and synaptonemal complex assembly. Located in chromosome. Is expressed in ovary; spermatid; spermatocyte; spermatogonium; and testis. Orthologous to human C1orf146 (chromosome 1 open reading frame 146).
Purification Method
Affinity purification
Gene ID
76421
RRID
AB_2862439
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat testis, using C1orf146 Rabbit pAb (CAB18804) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded rat testis using C1orf146 Rabbit pAb (CAB18804) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded mouse testis using C1orf146 Rabbit pAb (CAB18804) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
