The Calpain 2 Monoclonal Antibody (CAB4066) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity for detecting calpain 2 in human samples, making it ideal for use in Western blot applications.Calpain 2 is known to play a critical role in cell signaling, protein turnover, and cytoskeletal dynamics, making it a key player in processes such as cell migration, differentiation, and apoptosis. Dysregulation of calpain 2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and muscular dystrophies, making it a valuable target for research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Calpain 2 Monoclonal Antibody
SKU:
CAB4066
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0891
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CANP2, mCANP, CANPL2, CANPml, Calpain 2
Positive Sample:
HeLa, Mouse lung, Mouse brain, Mouse kidney, Rat lung, Rat brain, Rat kidney
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
80kDa
Observed MW:
80kDa
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
824
RRID
AB_2863180
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Calpain 2 Rabbit mAb (CAB4066) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Nobuhiro Morishima & Yoshihiro Ito et al.
Calpain-5 regulates muscle-specific protein expression and nuclear positioning during myoblast differentiatio
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