Multiplex Assays
Multiplex immunoassays by flow cytometry
Quantify up to 24 analytes from as little as 15 µL of sample on any flow cytometer — 450+ validated analytes across human, mouse, rat, NHP and canine, with free training and data analysis.
Choose your species
Ready-to-use, cross-reactivity-validated GeniePlex panels — pick a species to browse the full range, or build a custom panel.
Human
Th1/Th2/Th17, inflammation, chemokine & CD8 T-cell panels
View Human panels →24 panelsMouse
Th1/Th2/Th17, inflammation & chemokine panels
View Mouse panels →12 panelsCanine
Canine cytokine & inflammation panels
View Canine panels →5 panelsNon-Human Primate
NHP Th1/Th2/Th17 & IGF panels
View Non-Human Primate panels →4 panelsRat
Rat Th1/Th2/Th17 & cytokine-release panels
View Rat panels →Build yoursCustom Panels
Bespoke panels validated & delivered in 7–10 days
View Custom Panels panels →More data, less sample, less time
Up to 24 analytes
Quantify up to 24 targets simultaneously in one well.
Just 15 µL
As little as 15 µL of sample per well.
Any flow cytometer
No special instrument — run on any 488 nm (or dual 488/640 nm) cytometer.
High sensitivity
< 2 pg/mL for many analytes.
~4-hour protocol
Three short incubations, one read.
Validated
Every panel tested for cross-reactivity in-house.
Compatible sample types: Cell-culture supernatant, serum, plasma, saliva, cell & tissue lysates, BALF, pleural & peritoneal fluids and more.
Bead-based sandwich immunoassay
GeniePlex uses bead populations distinguished by size and fluorescence intensity, each conjugated with a specific capture antibody that traps its target analyte (e.g. a cytokine) from the sample.
Captured analyte is detected with a biotinylated detection antibody against a second epitope, followed by Streptavidin-R-phycoerythrin. A flow cytometer reads each bead’s identity and its PE signal, and concentrations are read off a standard curve — measuring up to 24 analytes in a single reaction.
Three short incubations, one read
The complete GeniePlex protocol runs in about 4 hours.
Capture (60 min)
Incubate sample with capture-antibody-conjugated beads, then wash.
Detect (30 min)
Add biotinylated detection antibody, then wash.
Read (20 min)
Add Streptavidin-PE, wash, resuspend and acquire on a flow cytometer.
Why multiplex?
Measuring many analytes at once means less sample, fewer plates, less time — with sensitivity that matches or beats singleplex ELISA, and excellent correlation with Luminex® xMAP technology.
Service & support
Custom panels in 7–10 days
Mix and match analytes for human, mouse, rat, NHP or canine — bespoke panels fully validated before they ship.
See recent custom panels →3 free training sessions
Live Zoom support to set up your cytometer, run standards & samples, and analyse your data.
Flow cytometer set-up →Free data analysis <12 h
Send us your FCS files and we return a full data report in under 12 hours.
GeniePlex FAQ →Trusted in peer-reviewed studies
Twelve published studies and preprints (2021–2025) explicitly cite GeniePlex multiplex immunoassays — across oncology, neuroinflammation, NAFLD/NASH, SARS-CoV-2, knee osteoarthritis and nanomedicine.
NK cell line expressing a DR5-specific TRAIL variant shows enhanced cytotoxicity in ovarian cancer
PROS1 and SARS-CoV-2 jointly regulate the monocyte inflammatory response
Mechanical actuation of implantable devices modulates the foreign body response
Biocompatibility screening of metal-organic framework nanomedicines
Intra-articular inflammatory biomarkers linked to sensitization in late-stage knee OA pain
Modelling fatty liver disease with mouse liver-derived multicellular spheroids
CK2 inhibition suppresses glial inflammation in the brain
Endothelial cells retain inflammatory memory through chromatin remodeling
Scaffold-mediated lentiviral delivery for in vivo cancer immunotherapy
Claudin-6 in breast cancer immunoediting and anti-tumour cytokine response
T-cell receptor signalling in engineered T cell populations
Custom rat 8-plex cytokine profiling in skeletal muscle pharmacology
Multiplex tools & resources
Not sure which panel you need?
Book a free consultation with a GeniePlex expert, or let our PhD-level team design a custom panel for your study.
Talk to our team