Glycerol Assay Kit
Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications.Assay Genie's glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to glycerol concentration in the sample.
ApplicationsFor quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.
Glycerol Assay Kit -Key Features
- Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate: 10 to 1000 uM (92 ug/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 for fluorimetric assays.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.
- Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Glycerol Assay Kit Information:
|Kit Includes:||Assay Buffer: 24 mL Enzyme Mix: 500 mL ATP: 250 mL Dye Reagent: 220 mL Standard: 100 mL 100 mM Glycerol|
|Kit Requires:||Pipeting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well plates and plate reader.|
|Method of Detection:||OD570nm, or FL530/585nm|
|Detection Limit:||2 uM|
|Samples:||Biological, food, beverage etc|
|Protocol Length:||20 min|
|Storage:||Store all components at -20°C|
|Shelf Life:||12 months|