Glycerol Assay Kit (BA0115)

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SKU:
BA0115
€601

Description

ELISA Kit Technical ManualMSDS

Glycerol Assay Kit

Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications.

Assay Genie's glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to glycerol concentration in the sample.

Applications

For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.

Glycerol Assay Kit -Key Features

  • Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate: 10 to 1000 uM (92 ug/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 for fluorimetric assays.
  • Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.
  • Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Glycerol Assay Kit Information:

Kit Includes: Assay Buffer: 24 mL Enzyme Mix: 500 mL ATP: 250 mL Dye Reagent: 220 mL Standard: 100 mL 100 mM Glycerol
Kit Requires: Pipeting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well plates and plate reader.
Method of Detection: OD570nm, or FL530/585nm
Detection Limit: 2 uM
Samples: Biological, food, beverage etc
Species: All
Protocol Length: 20 min
Size: 200 tests
Storage: Store all components at -20°C
Shelf Life: 12 months

 

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