Description
Human COVID Cytokine Release Syndrome (CRS) 16-plex
Analytes
IFNγ, IL-1RA, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, MCP-1, MIP-1α, RANTES and TNFα
Species Reactivity:
Human
Assay Type:
Multiplex
Detection Method:
Flow Cytometry
Assay Time:
2 hours
Product Overview
Cytokine Release Syndrome Multiplex Panel 9-plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFNγ, IL-1RA, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, MCP-1, MIP-1α, RANTES and TNFα on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 3 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Product Information
Reactivity:
|
Human
|
Buffers:
|
2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
|
Analytes:
|
IFNγ, IL-1RA, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, MCP-1, MIP-1α, RANTES and TNFα |
Lyophilized Standard Mix:
|
Human COVID-19 Multiplex Cytokine Immunoassay. One vial containing lyophilized recombinant IL-6, IL-10, IFN-gamma, CCL2/SYCA2/MCP-1, GM-CSF/CSF-2, TNF-alpha, IL-1beta, IL-2, and IL-8/CXCL8 |
Sample types:
|
Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
Standard dose recovery:
|
70-130% |
Intra-assay CV:
|
< 10% |
Inter-assay CV:
|
< 20% |
Cross-reactivity of analytes in the Panel:
|
Negligible |
Sample volume:
|
15 µL/test |