The [KO Validated] PARK7 Antibody (CAB0987) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, developed using rabbits, demonstrates high reactivity with human samples and has been rigorously validated for Western blot applications. By specifically binding to the DJ-1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for use in studies focused on neurodegenerative diseases, Parkinson's disease, and other disorders associated with DJ-1 dysfunction.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] PARK7 Antibody
SKU:
CAB0987
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.
Purification Method
Affinity purification
Gene ID
11315
RRID
AB_2757506
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] DJ-1/PARK7 Rabbit pAb (CAB0987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of lysates from wild type (WT) and DJ-1/PARK7 knockout (KO) 293T cells, using [KO Validated] DJ-1/PARK7 Rabbit pAb (CAB0987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
