L-Alanine Assay Kit (Colorimetric/Flurometric) (BA0073)
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L-Alanine Assay Kit - InformationThe L-Alanine assay kit provides a simple, direct and automation-ready procedure for measuring alanine concentration.This assay allows for flurometric and colorimetric detection of alanine in biological samples. Alanine is converted into pyruvate which can then be directly measured. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to the alanine concentration in the sample.
ApplicationsFor quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism.
L-Alanine Assay Kit- Key Features
- Sensitive and accurate. Linear detection range in 96-well plate: 1 to 200 alanine for colorimetric assays and 0.4 to 20 for fluorimetric assays.
L-Alanine Assay Kit - Data Sheet
|Kit Includes||Developer: 6 mL ALT Enzyme: 120 mL Dye Reagent: 120 mL Cosubstrate: 600 mL Alanine Standard: 400 mL|
|Kit Requires||Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.|
|Method of Detection||OD570nm, or FL530/585nm|
|Samples||Plasma, serum, urine, tissue and culture media|
|Protocol length||60 min|
|Storage||Store all kit components at -20 °C.|
|Shelf Life||6 months|