FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family. The FGFs family contains a broad range of proteins that are involved in diverse aspects of development, including cell proliferation, growth, and specialization. FGFs act on a variety of cell types to control many physiological activities, such as angiogenesis, cell development, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is produced in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b; 1c; 2c; 3c and 4. Human FGF-2/FGFb Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is ?10 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast growth factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Gly132-Ser288 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17.4 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl; 150mM NaCl; pH 7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
