The RARbeta Monoclonal Antibody (CAB4535) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in various experimental techniques such as Western blotting.RARβ is known to play a critical role in cell differentiation, proliferation, and apoptosis, making it a key player in various biological processes including embryonic development, immune response, and carcinogenesis. The ability to specifically target and detect RARβ using this antibody allows for more detailed investigations into its function and potential therapeutic applications in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RARbeta Monoclonal Antibody
SKU:
CAB4535
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1024
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. Alternate promoter usage and differential splicing result in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5915
RRID
AB_2863290
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RARβ Rabbit mAb (CAB4535) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using RARβ Rabbit mAb (CAB4535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using RARβ Rabbit mAb (CAB4535) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
