D-Sorbitol Assay Kit
SORBITOL (glucitol) is a sugar alcohol that is metabolized slowly in the human body. Sorbitol can be obtained from glucoseby reducing aldehyde group to a hydroxyl group. Accumulation of excessive sorbitol in erythrocytes, retinal cells, andSchwann cells has been associated with retinopathy, cataracts, peripheral neuropathy and diabetes. Sorbitol is madesolely from corn syrup, and found in fruits such as apples, pears, peaches, and prunes. It is widely used as a sugar substituteand as a laxative. It is also utilized in specialty culture media and in healthcare, food and cosmetic products. Sorbitol ismeasured in biological samples to monitor metabolic pathways and the progression of diabetes.The Assay Genie D-Sorbitol Assay Kit involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the sorbitol concentration.
ApplicationsFor quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism.
- Fast and sensitive. Linear detection range (20 uL sample): 5 to 1000 uM D-sorbitol.
- Convenient and high-throughput. Room temperature assay. No 37°C incubator required. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
D-Sorbitol Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL, NAD/MTT: 1 mL, Enzyme A: 120 uL, Enzyme B: 120 uL, Standard: 250 uL, 50 mM Sorbitol|
|Kit Requires:||Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader|
|Method of Detection:||OD565nm|
|Samples:||Biological (e.g. blood), food, beverage and agriculture samples.|
|Protocol Length:||30 min|
|Storage:||Store all components at -20°C upon receiving|
|Shelf Life:||6 months|