D- Mannitol Assay Kit (Colorimetric) (BA0133)

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ELISA Kit Technical ManualMSDS

D- Mannitol Assay

D-MANNITOL (C6H14O6) is a sugar alcohol used in dietary supplement, sweetener, intestinal permeability test for leaky gut, etc. It also serves as a coating for hard candies, dried fruits, and chewing gums due to its low ability to attract and hold water molecules. In addition, it is an osmoprotectant for plants and is used clinically in osmotherapy to reduce intracranial pressure.

This D-mannitol colorimetric assay kit is based on mannitol dehydrogenase catalyzed oxidation of D-mannitol, which generates D-fructose and NADH that reduces a formazan (MTT) dye. The intensity of product color, measured at 565 nm is directly proportional to D-mannitol concentration in the sample


For quantitative determination of D-mannitol in biological, food, beverage and agriculture samples

Key Features

  • Fast and sensitive. Use of 20 uL sample. Linear detection range 0.007 to 3 mM mannitol in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
  • High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

D-Mannitol Assay Information:

Kit Includes: Assay Buffer: 10 mL, Standard: 0.5 mL, 20 mM D-Mannitol, Enzyme: 120 uL
Kit Requires: Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates and plate reader.
Method of Detection: OD565nm
Detection Limit: 7 uM
Samples: Biological, food, beverage and agricultural products
Species: All
Protocol length: 30 min
Size: 100 tests
Storage: Store all components at -20°C upon receiving.
Shelf Life: 6 months