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Subcellular Fractionation Protocol - Mitochrondria, Nuclear, Cytosolic(Page)
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Beyond Burnout: How T Cell Exhaustion Hijacks Cancer Immunity and What We Can Do About It(Post)
Imagine a highly trained soldier, elite and effective, sent to the front lines of a relentless war. Day after day, they fight without rest, their supplies dwindle, and the enemy never stops coming. Ev
10 Steps of Glycolysis: Enzymes, Diagram & ATP Yield(Post)Metabolism · Biochemistry Guide 10 Steps of Glycolysis: Enzymes, Diagram & ATP Yield The complete ten-step pathway that converts one glucose m
Validated Multiplex Panels by Flow Cytometer(Page) 10 reasons why you should do a science outreach event(Post)I'll start this post off with a story. When I was half way through my PhD and I had found my passion for science communication, I approached my supervisor for the first time to ask “Can I ha
Microglial Functions Explained: Immunity, Inflammation & Neurodegeneration(Post)Neuroscience · NeuroimmunologyMicroglial Functions: Immunity, Inflammation & NeurodegenerationMicroglia are the resident immune cells of the central nervous system — dynamic sentinels that survey the
Innate Immunity: Components, Functions & First Line of Defence(Post)Immunology · Innate ImmunityInnate Immunity: Components, Functions & First Line of DefenceThe innate immune system is the body’s first line of defence — a fast, non-specific response built from physic
CRISPR-Cas Screening for Cell Viability(Post)Key Points Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a method of gene editing that uses t
BDNF in inflammation and neuropathologies(Post)BDNF Overview Brain-derived neurotrophic factor (BDNF) is a central mediator of neuroplasticity, a term used to describe the ability of neurons
Deprotonation: Unveiling the Chemistry Behind It(Post)Chemistry · Acid-BaseDeprotonation: Mechanism, pKa and Choosing the Right BaseDeprotonation is the removal of a proton from a molecule to give its conjugate base. Whether it happens, and how completel
p53 Protein: Mutations, Cancer Signalling & Tumour Suppression(Post)Cancer Biology · Cell Signallingp53: Structure, Signalling, Regulation and Mutation in Cancerp53 is the most frequently mutated gene in human cancer, and the reason is structural: a single tetrameric
Galectin Therapeutics(Post)Idiopathic Pulmonary Fibrosis Idiopathic Pulmonary Fibrosis (IPF) is a chronic, irreversible and ultimately fatal disease characterized by prog
Transformation Protocol – Sample Transformation Protocols(Post)Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact
3D Cell Culture: Assay Guide(Post)References Key Points Three-dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fa
chemokine receptors and ligands(Post)Chemokines are highly conserved cytokines that control the movement of cells around the body. All 45 members of the chemokine family can be divided into 4 subfamilies depending on th
Blood Coagulation Pathway: Factors, Cascade & Disorders [Guide](Post)Haematology · CoagulationBlood Coagulation Pathway: Factors, Cascade and DisordersThe coagulation cascade is usually taught as two pathways converging on a common one. That framework maps neatly onto
The role of IL-18 in health and disease(Post)Exploring IL-18: A key cytokine in inflammation and disease. Key Takeaways: IL-18, a pro-inflammatory cytokine, plays a pivotal role in immune responses, particularly in inducing Type II
HMGB1 in inflammation and cancer(Post)HMGB1 Functional Overview High mobility group box 1 ( HMGB1), a member of the high-mobility group (HMG) family, was first identified in the thymus in 1973 an
Cellular Respiration: Stages, ATP Production & Pathway Guide(Post)Metabolism · Biochemistry Guide Cellular Respiration: Stages, ATP Production & Pathway Guide How cells convert glucose and oxygen into ATP acr
Wnt Signalling Pathway in Immunity(Post)What is Wnt Signalling? The Wnt signalling pathway is an evolutionarily conserved pathway that plays critical roles in the regulation of determining cell f
Metabolic Exhaustion: How Mitochondrial Dysfunction Sabotages CAR-T Cell Therapy in Solid Tumors(Post)Imagine engineering a patient's own immune cells into precision-guided missiles against cancer—cells that can seek out and destroy tumors with remarkable specificity. This is the promise of CAR-T cell
Lactobacilli - Structure, Metabolism, and Testing(Post)Microbiology · ProbioticsLactobacilli: Structure, Metabolism, Identification and Probiotic UseLactobacilli are lactic acid bacteria found in fermented foods and across the human gastrointestinal, oral
Carbon Dioxide sensing in Immune Cells(Post)BRIEF HISTORY OF THE “GREEN” EVOLUTION OF OXYGEN AND CARBON DIOXIDE WITHIN OUR ATMOSPHERE Million years ago carbon dioxide (CO2) levels used to be conside
Validated GeniePlex Panels - Customer Applications(Page) Validated GeniePlex Panels(Page)