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Flow Cytometry · Cell SortingGuide to Fluorescence-Activated Cell Sorting (FACS)Fluorescence-activated cell sorting (FACS) is a specialised form of flow cytometry that physically isolates individual cells from a mixed sample based on their fluorescence and light-scattering properties. With sorting purities approaching 99.99%, it is one of the most powerful tools in modern cell biology, immunology and stem cell research.Explore flow cytometry antibodies →99.99%achievable sort purity1000sof cells sorted per second4+parameters measured at onceSinglecell resolutionKey TakeawaysFACS isolates individual cells with a purity approaching 99.99%.It sorts cells by size, granularity, surface markers and …

RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein from cultured cells. RIPA buffer cell lysis enables determination of protein concentration. RIPA buffer is an ideal cell lysis reagent since it contains three non-ionic and ionic detergents. Preserving protein:protein interactions When you need to preserve protein-protein interactions or to reduce denaturation its recommended to use a RIPA buffer recipe without SDS (ionic detergent) or Triton X-100 (non-ionic detergent). RIPA buffer recipes …

SARS-CoV-2 qPCR assay from Assay Genie Assay Genie have just launched a qPCR assay for the detection of SARS-CoV-2. The SARS-CoV-2 One-Step RT-qPCR Kit for Coronavirus Detection (Strain 2019-nCoV) can detect SARS-CoV-2 in a range of sample types including sputum; nasopharyngeal, oropharyngeal aspirates, washes or swabs; tracheal aspirates by qPCR. What is in the kit? The assay genie SARS-CoV-2 qPCR assay kit comes with enough reagents to run 100 reactions. This kit also comes in 5, 10 and 500 pack sizes. Each kit comes with the following reagents for SARS-CoV-2 detection. …