Assay Reagents & Solutions
Supporting reagents for flow cytometry and cell-based assays: staining and lysis buffers, viability dyes, cell stimulation reagents, T cell activation beads and conjugation reagents.
Cell staining buffer, RBC and ACK lysis buffers, intracellular fixation and permeabilisation, and a Foxp3 transcription factor staining kit.
Propidium iodide, 7-AAD, DAPI and Calcein AM solutions, and fluorescent dUTPs.
Human and mouse CD3/CD28 T cell activation beads, cell stimulation and protein transport inhibitor mixes, PE, APC and PerCP, SMCC-activated proteins and a BSA removal kit.
Intracellular cytokine staining needs three things: stimulation to induce cytokine production, a protein transport inhibitor to keep the cytokine inside the cell, and fixation and permeabilisation so that the antibody can reach it. Transcription factors such as Foxp3 sit in the nucleus and need a dedicated staining buffer set.
A viability dye should be included in most panels so that dead cells, which bind antibodies non-specifically, can be gated out.
Pick the dye that leaves your other channels free: PI and 7-AAD are read in the red and far-red, DAPI in the violet-excited blue channel.
When staining for intracellular cytokines, so that the cytokine accumulates in the cell instead of being secreted.
They are pre-activated for conjugation to molecules with free thiol groups, for building your own conjugates.