Coenzyme A (CoA) Assay Kit (Colorimetric/Fluorometric) (BA0093)
Coenzyme A Assay Kit - InformationAssay Genie's Coenzyme A Assay Kit method provides a simple, two-step and high-throughput assay for measuring coenzyme A. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the coenzyme A concentration in the sample.
ApplicationsFor quantitative determination of coenzyme A and evaluation of drug effects on coenzyme A metabolism.
Coenzyme A Assay Kit - Key Features
- Sensitive. Use 10 samples. Linear detection range: colorimetric assay 5 - 1000 , fluorimetric assay 3 - 100 CoA.
- Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.
Coenzyme A Assay Kit - Data Sheet
|Method of Detection||OD570nm, or FL530/585nm|
|Protocol Length||60 min|
|Shelf Life||6 months|
More DetailsCoenzyme Ais involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of coenzymes A most crucial roles is the carrying and transferring of acyl groups.