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Granulocytes Guide(Post)
Immunology · Myeloid CellsGranulocytes: Neutrophils, Eosinophils and BasophilsGranulocytes are the leukocytes with cytoplasmic granules and lobed nuclei — neutrophils, eosinophils and basophils. They
Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer(Post)Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Pancreatic canc
CIDE: The New Bioinformatics Engine Unmasking Secreted Proteins for Precision Immunotherapy(Post)For decades, the battle against cancer has focused on the cellular 'soldiers' and the genetic 'blueprints' within them. Yet, a vast, invisible world of communication exists between these cells—a secr
ELISA vs Western Blot: Which Technique Should You Choose?(Post)Written by Seán Mac Fhearraigh, PhD • Updated: 19 May 2026 • ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through
Understanding Blotchy Western Blots: Causes and Remedies(Post)Western blotting is a powerful and widely used technique in molecular biology, essential for detecting and quantifying specific proteins in complex biological samples. However, despite its popu
Loading Control Antibodies(Page)Loading Control Antibodies | GAPDH, \u03b2-Actin & Tubulin | Assay Genie
Comprehensive Guide to Mononuclear Cells(Post)Immunology · Cell IsolationPeripheral Blood Mononuclear Cells (PBMCs): Composition, Isolation and UseMononuclear cells are the leukocytes with a single, unlobed nucleus — lymphocytes and monocytes. Is
Guide to Western Blot Sample Preparation(Post)Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti
DNA Assembly Cloning(Page) IHC Guide(Page) The Strategic Role of Secondary Antibody Incubation Times in Immunodetection Techniques(Post)Immunodetection · ProtocolsSecondary Antibody Incubation: Times, Conditions and TroubleshootingThe secondary antibody step determines how much signal you get and how much background comes with it. Inc
Passaging Hybridoma Cells: A Detailed Guide(Post)Cell Culture · ProtocolsPassaging Hybridoma Cells: A Detailed GuideHybridomas are productive but temperamental. They grow in suspension, are sensitive to shear and to overgrowth, and — the problem tha
TA Cloning(Page) Immunohistochemical Staining, Antibodies & Buffers(Page) Transmission of SARS-CoV-2 (COVID-19) via Droplets and Aerosols (Post)Transmission of SARS-CoV-2 (COVID-19) via Droplets and Aerosols What is COVID-19? COVID-19, the disea
KO Validated Antibodies(Page)KO Validated Antibodies | Assay Genie
Time-Resolved Fluorescence (TRF): A Comprehensive Introduction(Post)Time-Resolved Fluorescence (TRF) technology is a cutting-edge analytical technique that has revolutionized the fields of biochemistry, immunology, and molecular biology. By offering a refined appr
Navigating the Intricacies of Intracellular Flow Cytometry: Key Considerations(Post)In the evolving landscape of cellular biology, intracellular flow cytometry (ICFC) stands out as a powerful technique for analyzing the complexities within cells. This advanced method extends the
Automation of MR-proANP and MR-proADM ELISA Kits on Dynex DS2 Instrument(Post)The Dynex DS2 platform fully automates the MR-proANP and MR-proADM ELISA assays, streamlining sample processing from loading to detection. Automation ensures consistent assay performance
Cytokine neutralisation network(Page) C reactive protein(Post)Biomarkers · InflammationC-Reactive Protein (CRP): Structure, Function and Clinical UseCRP is the most widely measured marker of inflammation in medicine — fast to rise, fast to fall, and entirely non
Cytokine JAK/STAT signalling(Page) Streptococcus pneumoniae: Pathogenesis, Diagnosis, and Treatment(Post)Microbiology · Bacterial PathogensStreptococcus pneumoniae: Pathogenesis, Diagnosis & TreatmentStreptococcus pneumoniae (the pneumococcus) is a Gram-positive, encapsulated bacterium that colonises the
Cystic Fibrosis: Causes, Inheritance, Treatment and Diagnosis(Post)What is Cystic Fibrosis? Cystic fibrosis (CF) is a genetic disorder that impairs the body's ability to produce sweat and thick mucus. CF causes mucus to build up in t
The Notch Signalling Pathway: Its Steps and Impacts(Post)Notch signalling pathway review The Notch signalling pathway is a highly conserved intercellular signalling pathway that regulates various cellular processes. The Notch receptor is a tr
The common characteristics of cancer | Assay Genie(Post)By Shane Houston, PhD Candidate Queen’s University Belfast Cancer is an age old adversary of the human race. This out-of-control growth of abnormal cells has been a shadow over human health t
KIR Blockade: Empowering NK Cells for Superior Tumor Clearance(Post)Introduction to KIR Blockade in Cancer ImmunotherapyNatural killer (NK) cells are crucial components of the innate immune system, responsible for recognizing and destroying virally infec
Hemocytometer Cell Counting Procedure(Post)Cell Culture · ProtocolsHaemocytometer Cell Counting: Procedure, Calculation and Common ErrorsA haemocytometer turns a known chamber volume into a cell concentration, and the whole method rests on fou
Site Directed Mutagenesis(Page)Site-Directed Mutagenesis | Protocol, Kits & Troubleshooting | Assay Genie
Prevention and Risk Mitigation: Strategies to Reduce Hantavirus Exposure in a Changing World(Post)As hantavirus cases continue to rise globally, the question becomes not just how to detect infections, but how to prevent them in the first place. Recent epidemiological investigations across Califor
Mycoplasma Troubleshooting Tips & FAQ(Page) Mouse Injection Guide: Intraperitoneal, Subcutaneous, Intramuscular & Intravenous Routes(Post)In Vivo Methods · ProtocolsMouse Injection Guide: Intraperitoneal, Subcutaneous, Intramuscular & Intravenous RoutesRoute choice and injection technique determine how much of a dose actually reaches it
Western Blot Protocol & Troubleshooting Guide(Page)Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie
Tocilizumab (Actemra®) in COVID-19 Treatment(Post)Tocilizumab (Actemra®) in COVID-19 Treatment What is Tocilizumab? Tocilizumab, also called atlizumab, is an immunosuppress
Prostate Cancer Markers : What to Look for in Your Test Results(Post)In this blog post, we will review prostate cancer markers and what you should look for in your results. Prostate antigen marker (PSA) and p63 are the most common markers found in pro
Immuno Oncology: If cancer was a pathogen, would a vaccine be able to destroy it?(Post)Thanks to the great intuitions of brilliant scientists like Louis Pasteur, Edward Jenner, Robert Koch, Emil von Behring, Gaston Leon Ramon, Albert Sabin, Jonas Salk in the past century, the
Growth Factors Can Cooperate to Promote Tumorigenesis(Post)Introduction Tumorigenesis, the process by which normal cells transform into cancer cells, is a multifaceted and complex event influenced by various internal and external factors. Among the
Tocilizumab: Advancing Therapeutics and Research with Biosimilars(Post)What You Need to Know About TocilizumabWhat is Tocilizumab?Tocilizumab is a monoclonal antibody that targets the interleukin-6 (IL-6) receptor, widely used for managing autoimmune and in
Serum vs Plasma: Key Differences & When to Use Each [Guide](Post)Sample Preparation · ImmunoassaySerum vs Plasma: Key Differences and When to Use EachSerum and plasma come from the same blood draw but are not interchangeable. One is collected after clotting, the ot
Red Blood Cell Peroxidase Interference in Competitive ELISA(Post)QUICK ANSWER A researcher ran a competitive 2,3-bisphosphoglycerate (2,3-BPG) ELISA on red blood cell (RBC) lysate. The standard curve was textbook, yet every lysate sample read at th
Data Science for Immunologists | Assay Genie(Post)By Dr. Niclas Thomas “Data science” is a recent addition to the vocabulary of analytics, and can mean many different things to different people. As far as
Immunopeptidomics: Mapping the Hidden Landscape of the Cancer Immunome(Post)Imagine a high-stakes game of hide-and-seek where the seeker is your immune system and the hider is a master of disguise: cancer. For decades, we believed the 'wanted' posters for tumor cells were li
Phosphorylation: Types, ATP Synthesis & Cell Signalling [Guide](Post)Biochemistry · Cell SignallingPhosphorylation: Types, ATP Synthesis and Cell SignallingAdding a phosphate group is how cells both store energy and switch proteins on and off. The same chemistry underl
Coeliac Disease Signaling – Review | Assay Genie(Post)By Ciara Coleman PhD General Introduction Coeliac Disease (CD) is defined as a common, chronic inflammatory disease
Sandwich ELISA: Step-by-Step Protocol & Troubleshooting(Post)Written by Seán Mac Fhearraigh, PhD • Updated: 19 May 2026 • ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant
Monocytes: An Overview (Post)Monocytes, as part of the innate immune system, act as the first line of defense against foreign invaders. One of their primary functions is phagocytosis, a crucial process in which they engulf
Scicomm (Science Communication) tips for beginners! (Post)Science communication (Scicomm) is currently on the up. As rebellion against the increasing distrust of science stemmed from bigoted and ignorant leaders, scientists are turning to s
Preeclampsia & immune cell regulation | Assay Genie(Post)What is preeclampsia? According to the World Health Organization (WHO), one in ten pregnant women is affected by hypertensive disorders, and preeclampsia a
Gut Inflammation and Inflammatory Diseases (Post)What is Gut Inflammation? The gut, or gastrointestinal (GI) tract is a long tube that starts from the mouth and extends to the anus. It includes various organs such
Mechanisms of HIV persistence during HAART(Post)HIV Virus: Replication Cycle and Pathogenesis in absence of HAART The human immunodeficiency virus (HIV) is a formidable pathogen that has posed a significant global
Androgenic steroid action in breast cancer(Post)By Rachel Bleach, PhD Student, RCSI By the age of 79 years, 1 in 14 women will develop breast cancer (Global Burden of Disease Cancer 2017). Beatson reported
Deprotonation: Unveiling the Chemistry Behind It(Post)Chemistry · Acid-BaseDeprotonation: Mechanism, pKa and Choosing the Right BaseDeprotonation is the removal of a proton from a molecule to give its conjugate base. Whether it happens, and how completel
Necrosis: Types, Causes, Symptoms & Prevention [2026 Guide](Post)Cell Biology · PathologyNecrosis: Types, Causes, Mechanisms and How It Is MeasuredNecrosis is cell death accompanied by membrane rupture and release of intracellular contents — which is what makes it
Carbon Dioxide sensing in Immune Cells(Post)BRIEF HISTORY OF THE “GREEN” EVOLUTION OF OXYGEN AND CARBON DIOXIDE WITHIN OUR ATMOSPHERE Million years ago carbon dioxide (CO2) levels used to be conside