Anti-Butyrly-HIST1H3A (K27) Antibody (PACO60564)

Antibody Name:	Butyrly-HIST1H3A (K27) Antibody (PACO60564)
Antibody SKU:	PACO60564
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, ICC, IF, ChIP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, ICC:1:20-1:200, IF:1:10-1:100
Species Reactivity:	Human
Immunogen:	Peptide sequence around site of Butyrly-Lys (27) derived from Human Histone H3.1

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h. All lanes: HIST1H3A antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/40000 dilution. Predicted band size: 16 kDa. Observed band size: 16 kDa.
	Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with PACO60564 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunocytochemistry analysis of PACO60564 diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms:

Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ

UniProt Protein Function:	H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:	P68431
NCBI GenInfo Identifier:	55977055
NCBI Gene ID:	8357
NCBI Accession:	P68431.2
UniProt Secondary Accession:	P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1,
UniProt Related Accession:	P68431
Molecular Weight:	15kDa
NCBI Full Name:	Histone H3.1
NCBI Synonym Full Names:	histone cluster 1 H3 family member h
NCBI Official Symbol:	HIST1H3H
NCBI Official Synonym Symbols:	H3/k; H3FK; H3F1K
NCBI Protein Information:	histone H3.1
UniProt Protein Name:	Histone H3.1
UniProt Synonym Protein Names:	Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name:	HIST1H3A

Antibodies	ELISA Kits
Anti-MonoMethyl-Histone H3-K27 Antibody (CAB2361)	Histone H3 Colorimetric Cell-Based ELISA Kit
Anti-Asymmetric DiMethyl-Histone H3-R26 Antibody (CAB2375)	Histone H3 (Phospho-Thr3) Colorimetric Cell-Based ELISA Kit
Anti-Asymmetric DiMethyl-Histone H3-R17 Antibody (CAB2421)
Anti-MonoMethyl-Histone H3-R17 Antibody (CAB3151)
Anti-Symmetric DiMethyl-Histone H3-R17 Antibody (CAB3152)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)