Anti-KEAP1 Antibody (CAB17061)

Antibody Name:	Anti-KEAP1 Antibody [KO Validated]
Antibody SKU:	CAB17061
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human KEAP1.

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Jurkat, Rat lung

Immunogen:	A synthetic peptide of human KEAP1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	9817
Uniprot:	Q14145
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	69kDa
Observed MW:	60-64KDa

Synonyms:

KEAP1, INrf2, KLHL19

Background:

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

UniProt Protein Function:	KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an Â¿ETGEÂ¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these Â¿ETGEÂ¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Transcription regulation; Motility/polarity/chemotaxis; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; microtubule organizing center; midbody; cytosol Molecular Function:protein binding; transcription factor binding Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of transcription factor activity; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of epidermal cell differentiation
NCBI Summary:	This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14145
NCBI GenInfo Identifier:	146345444
NCBI Gene ID:	9817
NCBI Accession:	Q14145.2
UniProt Related Accession:	Q14145
Molecular Weight:
NCBI Full Name:	Kelch-like ECH-associated protein 1
NCBI Synonym Full Names:	kelch like ECH associated protein 1
NCBI Official Symbol:	KEAP1
NCBI Official Synonym Symbols:	INrf2; KLHL19
NCBI Protein Information:	kelch-like ECH-associated protein 1
UniProt Protein Name:	Kelch-like ECH-associated protein 1
UniProt Synonym Protein Names:	Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19
Protein Family:	Kelch-like ECH-associated protein
UniProt Gene Name:	KEAP1
UniProt Entry Name:	KEAP1_HUMAN

	Western blot analysis of extracts of Jurkat cells, using KEAP1 antibody (CAB17061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts of Rat lung, using KEAP1 antibody (CAB17061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Western blot analysis of extracts from normal (control) and KEAP1 knockout (KO) 293T cells, using KEAP1 antibody (CAB17061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human colon using KEAP1 Rabbit pAb (CAB17061) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using KEAP1 Rabbit pAb (CAB17061) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-KEAP1 Antibody (CAB11258)	Human KEAP1(kelch-like ECH-associated protein 1) ELISA Kit
Anti-KEAP1 Antibody (CAB11484)
Anti-KEAP1 Antibody (CAB17062)
Anti-KEAP1 Antibody (CAB1820)
KEAP1 Antibody (PACO01930)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)