Succinyl-CoA Synthetase Activity Colorimetric Assay Kit (BN00824)

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BN00824
  • Succinyl-CoA Synthetase Activity Colorimetric Assay Kit (BN00824)
  • Succinyl-CoA Synthetase Activity Colorimetric Assay Kit (BN00824)
  • Succinyl-CoA Synthetase Activity Colorimetric Assay Kit (BN00824)
$997

Description

ELISA Kit Technical ManualMSDS

Succinyl-CoA Synthetase Activity Colorimetric Assay Kit

Succinyl-CoA Synthetase (SCS, also called Succinyl-CoA ligase, Succinate Thiokinase) (EC 6.2.1.5) is a critical enzyme in the citric acid cycle and an important metabolic intermediate for porphyrin, heme and ketone body biosynthesis. It is located in the mitochondrial matrix and is a heterodimer composed of one and one Beta subunit. In humans, Succinyl-CoA Synthetase deficiency causes the build-up of lactic acid leading to lactic acidosis, which can be fatal in infants. Measurement and analysis of SCS activity is useful for both mechanistic studies as well as for diagnostic purposes. In Assay Genie's Succinyl-CoA Synthetase Activity Assay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA. Succinyl-CoA reacts with the Developer to form a colored product with strong absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. It can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.

Figure: (a) NADH Standard Curve; (b) Succinyl-CoA Synthetase activity in mitochondria prepared from bovine heart, yeast (P.pastoris) and MCF-7 cells and in Jurkat cell lysate; (c) Succinyl-CoA Synthetase specific activity in mitochondria prepared from yeast (P. pastoris, 1.14 µg), bovine heart (1.1 µg) and MCF-7cells (0.5 μg), and in Jurkat cell lysate (25 μg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00824

Size

100 assays

Detection Methods

Absorbance (OD 450 nm).

Applications

  • Measurement of Succinyl-CoA Synthetase activity in various tissues/cells
  • Analysis of cell signaling pathway

Features and Benefits

  • Simple & Rapid Protocol
  • High-Throughput adaptable
  • This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.

Kit Components

  • SCS Assay Buffer
  • SCS Substrate Mix (Lyophilized)
  • SCS Enzyme Mix (Lyophilized)
  • SCS Developer (Lyophilized)
  • NADH Standard (Lyophilized)
  • SCS Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.
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