- Metabolism Assays
- Acetylcholinesterase Inhibition Assay Kit (Colorimetric) (BA0160)
Acetylcholinesterase Inhibition Assay Kit
ACETYLCHOLINESTERASE (EC 188.8.131.52, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Inhibition of the enzyme leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. AChE inhibition is an important target for the management of Alzheimer's disease. In addition to Alzheimer's disease, AChE inhibitors have been useful in the diagnosis or treatment of diseases such as glaucoma, myasthenia gravis, bladder distention, and more.Assay Genie's Acetylcholinesterase Inhibitor Assay is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5'-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412nm, is proportionate to the enzyme activity in the sample.
ApplicationsHTS for inhibitor screening and evaluation of acetylcholinesterase inhibitors.
- Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at 37°C.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Acetylcholinesterase Inhibition Assay Kit Information:
|Kit Includes:||Assay Buffer (pH 7.5): 30 mL, DTNB: 60 uL, Substrate (100mM): 500 uL|
|Kit Requires:||Purified AChE and if desired a control AChE inhibitor. Pipeting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, and plate reader.|
|Method of Detection:||OD412nm|
|Protocol Length:||10 min|
|Storage:||Store the substrate and DTNB at -20°C and all other components at room temperature upon receiving.|
|Shelf Life:||12 months|