Anti-Mouse CD252 (OX40L) In Vivo Antibody

Product Name:	Anti-Mouse CD252 (OX40L) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0316
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	RM134L
Protein:	CD252
Product Type:	Monoclonal Antibody
Synonyms:	OX 40 Ligand, OX40L, CD134L, TNFSF4
Isotype:	IgG2b k
Reactivity:	Mouse
Applications:	B, FC, IHC FF, In Vivo, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	B, FC, IHC FF, In Vivo, WB
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone RM134L recognizes an epitope on mouse CD252.
Antigen Distribution:	CD252 is expressed on activated B cells and antigen presenting cells.
Immunogen:	Rat NRK-52E cell line transfected with mouse OX-40L gene
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.

Protein:	CD252
Function:	B-cell/T-cell interaction, costimulation
Ligand/Receptor:	OX40(CD134)
Research Area:	Costimulatory Molecules, Immunology
Shipping:	2-8°C
NCBI Gene Bank ID:	22164
UniProt:	CD252