QCA (Quinoxaline-2-carboxylic acid) ELISA Kit ) (FSES0058)



Technical Manual

QCA (Quinoxaline-2-carboxylic acid) ELISA Kit )

Kit Name: QCA (Quinoxaline-2-carboxylic acid) ELISA Kit )
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Quinoline-2-Carboxylic acid (QCA) in samples, such as muscle, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, QCA in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-QCA antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of QCA. The concentration of QCA in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.1 ppb (ng/mL)
Reaction Mode: 25°C,30 min~15 min
Detection Limit: Muscle--- 1 ppb
Cross Reactivity: Quinoline -2-Carboxylic Acid (QCA) --- 100%; Desoxycarbadox (DCBX) --- <0.1%; 3-Methylquinoline-2-Carboxylic Acid (MQCA) ---78%
Sample Recovery Rate: 90%±30%.
Kits Components:
Item Specifications
ELISA Microtiter Plate 96 wells
Standard Liquid 1 mL each<         (0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)
HRP Conjugate Dilution 7 mL
11×Concentrated HRP Conjugate 1 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20×Concentrated Wash Buffer 25 mL
5×Concentrated Sample Solution 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Homogenizer, Oscillators, Centrifuge, Nitrogen evaporators,Water bath.
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: H2SO4, Ethyl acetate, N-hexane.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
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