Hormone Peptide & Small Molecule ELISA Kits

ELISA Genie hormone peptide and small molecule ELISAs are a form of quantitative ELISA kits for peptides, hormones, amino acids, vitamins and other small molecules. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a competitive ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

  • Validated ELISA kits
  • Wide range assays
  • Normalization of data using standard curve
  • Low coefficient of variability (CV%)
  • High linearity
  • High recovery in Spike/Recovery assays

Features & Benefits

  • Big Choice - Allow for the quantitative measurement of a vast range of analytes including amino acids, peptides, proteins, lipids, vitamins, neurotransmitters, and small molecules.

  • Sensitive - Measure umol and picogram levels of many analytes.

  • Versatile - A variety of samples can be tested e.g. serum, plasma, saliva, urine, cell culture supernatant, tissue samples and other related supernatants.

  • Convenient - Ready-to-use 96-well kits for rapid analysis in most species. Additionally, kits are compatible with routine laboratory and HTS formats: Assays can be performed in microplates.

Figure 1. 96-well format of hormone peptide & small molecule ELISA kits

Kit Principle

These ELISA kits have been created based on the competitive binding enzyme immunoassay technique. The microtiter plate provided within these kits have been pre-coated with the analyte.

During the reaction, the analyte in the sample or standard competes with a fixed amount of biotin-labeled analyte for sites on an antibody specific to the analyte. Excess conjugate and unbound sample or standard are washed from the plate.

Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the analyte in the samples is then determined by comparing the O.D. of the samples to the standard curve.