The ACh (Acetylcholine) ELISA Kit from Assay Genie is specifically designed for the accurate detection of acetylcholine levels in various biological samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for researchers in a wide range of fields.Acetylcholine is a neurotransmitter that plays a crucial role in various physiological processes, including muscle contractions, memory, and learning. Dysregulation of acetylcholine levels has been associated with various neurological disorders, making it a valuable biomarker for studying these conditions.Whether you are studying the nervous system, neuropsychiatric disorders, or muscle physiology, the ACh ELISA Kit from Assay Genie provides a reliable and efficient platform for accurate measurement of acetylcholine levels. Order yours today and take your research to the next level.
Product Name:
ACH (Acetylcholine) ELISA Kit
SKU:
UNES00050
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
