The VD (Vitamin D) ELISA Kit is a highly reliable and accurate tool for the quantification of vitamin D levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing consistent and precise results for various research applications related to vitamin D analysis.Vitamin D is a critical nutrient that plays a vital role in bone health, immune function, and overall well-being. Deficiencies in vitamin D have been associated with a range of health issues, including osteoporosis, cardiovascular diseases, and autoimmune disorders. Therefore, monitoring vitamin D levels is essential for understanding and addressing these health concerns.Whether studying the effects of vitamin D supplementation, evaluating its impact on disease progression, or exploring its role in various physiological processes, the VD ELISA Kit is an invaluable tool for researchers seeking to unravel the complexities of vitamin D metabolism and its implications for human health.
Product Name:
VD (Vitamin D) ELISA Kit
SKU:
UNES00009
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
