The 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit is a powerful tool for the accurate measurement of 1,25-dihydroxyvitamin D3 levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.1,25-Dihydroxyvitamin D3, also known as calcitriol, is the active form of vitamin D in the body and plays a crucial role in calcium metabolism, bone health, and immune function. Abnormal levels of 1,25-dihydroxyvitamin D3 have been linked to various disorders including osteoporosis, autoimmune diseases, and certain types of cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its high performance and accuracy, the DHVD3 ELISA Kit is an essential tool for researchers and clinicians looking to investigate the role of 1,25-dihydroxyvitamin D3 in health and disease.
Product Name:
DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit
SKU:
UNES00012
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
