The 6-Keto PGF1a (6-Keto Prostaglandin F1a) ELISA Kit is a reliable and accurate tool for detecting levels of 6-Keto PGF1a in various biological samples. This kit offers high sensitivity and specificity, ensuring consistent and reproducible results for a wide range of research applications.6-Keto PGF1a is a metabolite of prostacyclin, a key player in the regulation of inflammation and vascular tone. It is known to have important implications in conditions such as cardiovascular diseases, thrombosis, and inflammatory disorders. Monitoring 6-Keto PGF1a levels can provide valuable insights into these physiological and pathological processes, making this kit essential for researchers studying these areas and developing potential therapeutic interventions.
Product Name:
6-keto-PGF1a (6-keto-prostaglandin F1a) ELISA Kit
SKU:
UNES00035
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
91-106
97-111
89-103
Average (%)
98
103
96
1:4
Range (%)
89-103
90-104
95-108
Average (%)
95
96
101
1:8
Range (%)
88-102
88-102
98-116
Average (%)
94
93
106
1:16
Range (%)
91-105
92-109
100-116
Average (%)
96
100
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
83-97
90
Cell Culture Media (n=8)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.15
149.6
445.39
49.91
144.62
480.51
Standard deviation
2.75
10.28
23.96
3.08
11.7
34.4
C V (%)
5.07
6.87
5.38
6.17
8.09
7.16
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 50 μL
Specificity:
This kit recognizes Universal 6-keto-PGF1a in samples.No significant cross-reactivity or interference between Universal 6-keto-PGF1a and analogues was observed
