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    p38 MAPK Signaling Review(Post)

    The p38 MAPK signaling pathway is an intracellular signaling pathway that plays a crucial role in cellular responses to various stressors and inflammatory stimuli. It is a part of the larger MAPK ...

    MAPK Signaling: Unraveling the Pathway of Mitogen Stimulation(Post)

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    Interferons(Post)

    Interferons (IFNs) are a group of signalling proteins that are produced and released from cells in response to viral infection and other stimuli such as normal and tumour cell survival and ...

    MAPK Oxidative Stress Pathway: A Journey through Cellular Signaling(Post)

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    How to Thaw Cells: Best Practices for Cell Culture Success(Post)

    Cell culture techniques are fundamental to biomedical research, providing crucial insights into cellular functions, drug discovery, and disease mechanisms. A critical step in cell culture is the ...

    Fenofibrate Usage for Diabetes Damage(Post)

    Fenofibrate, also known as FEN, is a drug that is used to lower cholesterol levels. It works by blocking the absorption of dietary cholesterol in the small intestine and therefore, re ...

    MAPK Signaling in Inflammatory Cytokines Pathways(Post)

    The Mitogen-Activated Protein Kinase (MAPK) pathway is a pivotal signaling cascade that plays a crucial role in mediating cellular responses to various external stimuli. This pathway is intricate ...

    SOCS proteins: Manipulating the immune response(Post)

    Katherine Edwards, PhD Student, Queen’s University Belfast Cytokines play a vital role in immune responses enabling cross-talk between different cell types ...

    Boiling Proteins for Western Blotting: Optimal Conditions and Best Practices(Post)

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    Guide to Bacterial Transformation: The Science of Genetic Manipulation(Post)

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    NLRP3 Inflammasome & Cytokine Secretion(Post)

    Nod-like receptors Nod like receptors (NLRs) are a versatile family of relatively recently discovered intracellular receptors which have a b ...

    Decoding the Language of Cells: Chemokine Signaling Pathways Unveiled(Post)

    Chemokines are small signaling proteins that play a crucial role in the immune system by directing the movement of cells, orchestrating immune responses, and maintaining tissue homeostasis. Th ...

    VEGF: Inflammation and Tumourigenesis(Post)

    What is VEGF? Vascular Endothelial Growth Factors (VEGFs) are a group of homodimeric polypeptides, and are master regulators of vascular development, maintenance and ...

    The role of IL-18 in health and disease(Post)

    Exploring IL-18: A key cytokine in inflammation and disease. Key Takeaways: IL-18, a pro-inflammatory cytokine, plays a pivotal role in immune responses, particularly in inducing Type II ...

    What I learnt from meeting 1000+ researchers(Post)

    Over the past few years I have traversed through academia in a few different guises, first as a PhD student for about 5 years, then onto the traditional route of a post-doc, followed by a sale ...

    ST2 and IL33(Post)

    ST2, a member of the IL-1R Subfamily ST2 is a member of the IL-1R subfamily and was identified in 1989 [1]. The ST2 gene is located on chromos ...

    Bullying in the Laboratory and workplace(Post)

    The lab can be one of the greatest places in the world to make live long friends. Spending countless hours in a tissue culture room late into the middle of the night, sharing similar frustrati ...

    Ras signaling pathway(Post)

    A wide variety of cell surface receptors activate Ras GTPase, including the tyrosine receptor linked to the epidermal growth factor receptor (EGFR) (Wells 1999). Upon receptor stimulation, son ...

    The Notch Signalling Pathway: Its Steps and Impacts(Post)

    Notch signalling pathway review The Notch signalling pathway is a highly conserved intercellular signalling pathway that regulates various cellular processes. The Notch receptor is a t ...

    HMGB1 in inflammation and cancer(Post)

    HMGB1 Functional Overview High mobility group box 1 ( HMGB1), a member of the high-mobility group (HMG) family, was first identified in the thymus in 1973 a ...

    Converting RPM to G-force in Centrifugation(Post)

    Centrifuges play a pivotal role in scientific research and various industries, enabling efficient separation and isolation of components within a sample. Understanding the relations ...

    Christmas Lab Party Competition | Assay Genie(Post)

    We know it gets tiring being in the lab every day, especially with the evenings getting darker and gloomier. So, to get you in the festive spirit, we're holding a competition to win money to t ...

    Data Science for Immunologists | Assay Genie(Post)

    By Dr. Niclas Thomas “Data science” is a recent addition to the vocabulary of analytics, and can mean many different things to different people. As f ...

    Keep your Lab Book up to Date(Post)

    When PhDs and Post-Docs start in new lab they have the best intentions to keep their lab books up to date with notes, new research methods, data, and protocol information. These best efforts u ...

    How to Choose a Secondary Antibody(Post)

    The selection of a secondary antibody is a critical step in the experimental design of various immunodetection methods, including Western Blot, ELISA, immunohistochemistry (IHC), and immunofluore ...

    Top 10 Universities in the UK(Post)

    Before I even started my PhD I was already thinking about carrying out PostDoc in a foreign country and had my sights set on going to the US. I had previously carried out a degree in Genetic ...

    OKRs for Researchers(Post)

    Objectives & Key Results Objectives & Key Results (OKRs) is a framework for setting and tracking goals. At Assay Genie, we've tailored this approach to sui ...

    What is RNAi? All You Need to Know(Post)

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    Department of Oncology Retreat 2025(Post)

    Department of Oncology RetreatAssay Genie was honoured to sponsor and participate in the inaugural 2025 Department of Oncology Retreat, hosted by the University of Lausanne and Lausanne ...

    Passaging Hybridoma Cells: A Detailed Guide(Post)

    Hybridoma cells are essential tools for producing monoclonal antibodies and are derived by fusing B lymphocytes (antibody-producing cells) with myeloma cells (cancer cells) to achieve bo ...

    Cortisol and the Immune Response(Post)

    Endocrinology · ImmunologyCortisol and the Immune ResponseCortisol is the body’s primary stress hormone — a glucocorticoid released by the adrenal gland that powerfully shapes the immune system. Acti ...

    Introducing BioSymposia: The Gathering Place for Science(Post)

    Announcement · Powered by Assay GenieIntroducing BioSymposia: The Gathering Place for ScienceWe’re excited to announce the launch of BioSymposia, our new sister platform and a free home for sc ...

    What is CAR T-cell therapy?(Post)

    CAR T-cell therapy CAR (Chimeric Antigen Receptor) T-cell Therapy is a type of cancer treatment. CAR T-cells are made in the laboratory and they have an artificially created receptor on th ...

    Scicomm (Science Communication) tips for beginners! (Post)

    Science communication (Scicomm) is currently on the up. As rebellion against the increasing distrust of science stemmed from bigoted and ignorant leaders, scientists are turning to ...

    COVID-19 Workplace Testing(Post)

    Workplace Testing for COVID-19 Corporate Testing for COVID-19 The impact of COVID-19 on the econo ...

    How to improve your relationship with your PI or Professor(Post)

    After probably a round or two of interviews and a PhD in your pocket you would think that proving yourself in your new lab would not be an issue. After all, they did hire you because you were ...

    Western Blot Training: A Comprehensive Guide(Post)

    Western blotting, a cornerstone technique in molecular biology, biochemistry, and immunogenetics, is pivotal for detecting specific proteins in a sample. Its applications span various scientific ...

    Prostate Cancer Markers : What to Look for in Your Test Results(Post)

    In this blog post, we will review prostate cancer markers and what you should look for in your results. Prostate antigen marker (PSA) and p63 are the most common markers found in pr ...

    Auto-inflammatory diseases and genetics (SAIDs)(Post)

    Shelly Pathak PhD candidate, University of Leeds SAIDs Systemic Auto-inflammatory diseases (SAIDs) have been defined as a group of mainly inherited disorde ...

    Freezing Stimulated T Cells: A Detailed Guide(Post)

    Cryopreservation of stimulated T cells is a common technique in immunology research, allowing for long-term storage while maintaining their functional viability. Stimulated T cells—espec ...

    Avizakimab: Unveiling the Role of Anti-CD47 in Cancer Research(Post)

    Quick Facts About AvizakimabWhat is Avizakimab?Avizakimab is a monoclonal antibody designed to target the CD47 protein, a key checkpoint in immune regulation, particularly in cancer the ...

    Histamine Receptors: Gatekeepers of Immunological and Neurological Responses(Post)

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    COVID-19 Antibody Testing(Post)

    COVID-19 Antibody Testing Antibody Production in COVID-19 COVID-19, the novel coronavirus disease ...

    Follicle Stem Cell Behaviour(Post)

    David Melamed, Phd Candidate, Colombia University When I started in the Kalderon Lab five years ago, I had no idea that our work would lead us to drastica ...

    CV writing tips for scientists(Post)

    Making the switch! This article provides some help on CV writing tips for scientists for PhDs and PostDocs looking for jobs outside of academia and what they might need to ...

    Cancer & Tumour Suppressors – Mini Review(Post)

    Cancer DevelopmentCancer is a group of diseases that involves abnormal cell growth resulting in malignant tumours/ malignant neoplasms. Cancer is a leading cause of morbidity, with 14 million n ...

    Leukemia Inhibitory Factor: An Overview (Post)

    Leukemia Inhibitory Factor and JAK-STAT Signalling Pathway Leukemia inhibitory factor (LIF) is a cytokine that belongs to the interleukin-6 (IL-6) family of cytokines. Other members of ...

    Biofilms: The Bacteria Invaders You Can't Ignore(Post)

    If you've ever had a urinary tract infection, you know all about bacteria. These tiny organisms can invade your body and cause all sorts of problems. But what you may not know is th ...

    Metabolism or pluripotency – which regulates which? A chicken or the egg story.(Post)

    By Sophie Arthur How many diseases and conditions do you think exist in this world? Thousands? Hundreds of thousands? Maybe even millions? Unfortunately, I don’t know the answer. But what ...

    Beyond Burnout: How T Cell Exhaustion Hijacks Cancer Immunity and What We Can Do About It(Post)

    Imagine a highly trained soldier, elite and effective, sent to the front lines of a relentless war. Day after day, they fight without rest, their supplies dwindle, and the enemy never stops coming. E ...

    Types of ELISA: Direct, Indirect, Sandwich & Competitive Compared(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiple ...

    10 reasons why you should do a science outreach event(Post)

    I'll start this post off with a story. When I was half way through my PhD and I had found my passion for science communication, I approached my supervisor for the first time to ask “Can I h ...

    Advice and Guidelines from The Jack Ma Foundation for the Treatment and Prevention of COVID-19(Post)

    COVID-19 Prevention and Treatment What is COVID-19? COVID-19 is an infectious disease caused by the cor ...

    Phosphatases and PTP1B – Mini review(Post)

    IntroductionThe human kinome is estimated to contain 518 genes, in comparison to the estimated 180 genes that comprise the phosphatome (Arena et al., 2005). These kinase genes represent a signi ...

    ​TNF alpha & Inflammation(Post)

    Immunology · Cytokine SignallingTNF-alpha and Inflammation: Structure, Signalling & TherapyTumour necrosis factor alpha (TNF-α) is one of the most important pro-inflammatory cytokines in the body ...

    TUNEL staining : The method of choice for measuring cell death(Post)

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    Tigatuzumab: Advancing Cancer Research with Targeted Therapies(Post)

    Quick Facts About TigatuzumabWhat is Tigatuzumab?Tigatuzumab is a monoclonal antibody that targets the death receptor 5 (DR5) pathway, inducing apoptosis in cancer cells.What is the mec ...

    The Role of Tumor Suppressor Genes in Cancer: Knudson Hypothesis & Oncogenes(Post)

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    Exosomes - Discovery, Cargo, Biogenesis & Functions(Post)

    by Ryan Wallis, PhD student, QMUL, Blizard Institute The microscopic world of cell biology was first explored by Robert Hooke in the 17th century and published in his work, Microgr ...

    Build a science blog to progress your career(Post)

    What do scientists consider their skill set when looking for jobs outside of academia? Mine where hard to sum. I was a PhD in cell biology. I could Western Blot, I had used thousands of ant ...

    Ferroptosis: A New Frontier in Cancer Therapy(Post)

    For decades, the primary strategy in cancer treatment has been to trigger apoptosis, a form of programmed cell death, in malignant cells. However, many cancers develop resistance to apoptosis-induci ...

    Multiple Sclerosis and Stem Cells(Post)

    By Sonika Singh PhD candidate, University of Nottingham A Devastating Disease Multiple Sclerosis (MS) is a chronic and inflammatory disease ba ...

    HERV-K group of endogenous retroviruses and cancer(Post)

    In 2006, upon completion of the Human Genome Project, it was discovered that nearly 8% of the human genome is made up of viral DNA. These viral remnants are composed of ancient germ ...

    The common characteristics of cancer | Assay Genie(Post)

    By Shane Houston, PhD Candidate Queen’s University Belfast Cancer is an age old adversary of the human race. This out-of-control growth of abnormal cells has been a shadow over human health ...

    The Powerhouse of Immunity: How Mitochondrial Fitness Fuels the Fight Against Cancer(Post)

    Why do powerful cancer immunotherapies work wonders for some patients but fail for others? The answer may lie not just in the cancer cells themselves, but in the energy levels of our own immune warri ...

    Transduction vs Transfection: Understanding Gene Delivery Techniques(Post)

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    ELISA vs Western Blot: Which Technique Should You Choose?(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is f ...

    3 Reasons to Publish in Open Access Journals(Post)

    Over the past few years momentum has gained in the scientific publishing community for researchers to publish their data in open access scientific journals. Open access to published research o ...

    Transformation Protocol – Sample Transformation Protocols(Post)

    Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bac ...

    Toll-Like Receptor Signaling Pathways: A Key to Innate Immunity(Post)

    In the intricate landscape of the immune system, toll-like receptors (TLRs) play a fundamental role in the first line of defense against pathogens. These receptors, essential components of the in ...

    The Strategic Role of Secondary Antibody Incubation Times in Immunodetection Techniques(Post)

    Secondary antibodies are pivotal in the detection of target antigens in various immunodetection assays such as Western blotting, ELISA (Enzyme-Linked Immunosorbent Assay), and immunohistochemistr ...

    8 Steps to Mindfulness in the Laboratory(Post)

    Stress and anxiety are a normal part of our daily lives and impact everything we do from our relationships with loved ones to our performance in the laboratory. Like any scientist, I experienced ...

    Validation of MycoGenie Rapid Mycoplasma Detection Kit - A highly sensitive visual determination method for Mycoplasma detection.(Post)

    The MycoGenie Rapid Mycoplasma Detection Kit enables the detection of 28 Mycoplasma species in under 1 hour using a simple visual determination method, eliminating the need for expensiv ...

    29 PhD Thesis Writing Tips(Post)

    Explore 29 essential tips for crafting a compelling and well-structured PhD thesis, blending effective writing practices with time management and academic rigor for a successful dissertation jour ...

    Research Universities in Ireland(Post)

    Since I am from Ireland I thought I would give as comprehensive view as possible for Research Universities in Ireland. By now you are probably considering looking for a PhD or PostDoc posit ...

    A Quick Guide to Myelin(Post)

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    A Quick Guide To Mycoprotein(Post)

    Mycoprotein is an alternative source of protein that can be used in place of meat. It is made from a fungi called Fusarium venenatum, and is consumed by vegetarians and vegans as a meat su ...

    Choosing a Fluorescent Protein: A Comprehensive Guide (Post)

    Fluorescent proteins (FPs) have revolutionized the field of molecular and cellular biology, allowing scientists to visualize and track biological processes in live cells with unprecedented clarit ...

    Fibroblast Markers(Post)

    Fibroblasts, essential components of connective tissues, play vital roles in maintaining tissue structure and function. Their remarkable ability to produce and organize the extracellular matrix ...

    Hybridoma Technology: Revolutionizing Antibody Production(Post)

    Immunology · Antibody ProductionHybridoma Technology: How Monoclonal Antibodies Are MadeHybridoma technology, pioneered by Georges Köhler and César Milstein in 1975, is the method that made monoclona ...

    TGF-β/SMAD signalling(Page)

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    Cell-Based ELISA Kits (Page)

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    Citations(Page)

    Product CitationsMore than 1,000 cited products in scientific journalsWe're not just any scientific company; our products have made waves in the scientific community. From groundbreaking ...

    Antibodies(Page)

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    Custom ELISA Kits(Page)

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    Oxidative Stress Assays(Page)

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    Ferroptosis Assays(Page)

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    Loading Control Antibodies(Page)

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    Tunel 1-Step Assay Kits(Page)

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    Custom Antibody(Page)

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    SuperSet DIY Development ELISA Kits(Page)

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    Anti-Tag Antibodies(Page)

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