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Human IL-4 ELISpot Pair

SKU:
HUDC0172
Product type:
ELISpot
Reactivity:
Human
€670
Frequently bought together:

Description

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Human IL-4 ELISpot Pair

Assay Genie ELISpot is a highly specific immunoassay for the analysis of IL-4 production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of IL-4 producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted IL-4 (qualitative analysis) and single cells that produce IL-4 (quantitative analysis). Cell secreted IL-4 is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured IL-4 is revealed by tracer antibodies and appropriate conjugates.