Mouse Visfatin Recombinant Protein (CPRT1093)

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CPRT1093
€278

Description

Recombinant Protein DatasheetAntibody MSDS

Visfatin Mouse Recombinant Protein

Name:Visfatin Mouse
Synonyms:PBEF, Pre-B cell colony-enhancingfactor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.
Introduction:Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus (T2DM). Adipose tissue produces several proteins (adipocytokines) such as leptin, adiponectin, resistin, tumor necrosis factor–?, and IL–6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established. Visfatin, also known as pre–B cell colony–enhancingfactor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL–7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, includinghumans and the mouse. It has been postulated to play a role in innate immunity.Visfatin exerts insulin–mimetic effects that are dose–dependent and quantitatively similar to those of insulin in stimulatingmuscle and adipocyte glucose transport, and in inhibitinghepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose–dependent fashion. In keepingwith its insulin–mimetic effects, visfatin was as effective as insulin in reducinghyperglycemia in insulin–deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causingreceptor phosphorylation and the activation of downstream signalingmolecules. However, visfatin and insulin did not compete for bindingto the insulin receptor, indicatingthat the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.
Description:Visfatin Mouse Recombinant produced in E.Coli is a single, non–glycosylated, polypeptide chain (aa 1–491) containing a 20 aa His tagand having511 amino acids. The total molecular mass is 57kDa. The Visfatin is purified by proprietary chromatographic techniques.
Source:Escherichia Coli.
Pack size:25µg
Physical Appearance:Sterile Filtered colorless 1mg/ml solution.
Formulation:The protein solution contains 1x PBS pH–7.4.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time.For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greater than 90.0% as determined by SDS–PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECREKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERGWNYILEKYDgHLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPITVATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKgYKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLgVNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHTVFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Additional Information

Product type:
Recombinant Protein
Reactivity:
Mouse
Host Species:
E.Coli
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