MycoGenie MycoPlasma Elimination Kit

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SKU:
MORV0012
€189

Description

ELISA Kit Technical Manual

MycoGenie Mycoplasma Elimination Kit - Introduction

MycoGenie MycoPlasma Elimination Kit is designed to eliminate Mycoplasma from contaminated cell culture media and serum.

Unlike conventional antibiotics used to treat mycoplasma contamination, this kit removes mycoplasma by disrupting membrane structure allowing elimination of even antibiotic-resistant mycoplasma. This is a much more effective method of eliminating mycoplasma versus other common methods that simply inhibit proliferation. It is also very effective at removing Gram-negative and positive bacteria. This kit successfully eliminates intracellular and extracellular mycoplasma after a 3-7 day treatment protocol. Furthermore, it is non-toxic to cells and has been shown to kill most mycoplasma species. The MycoGenie MycoPlasma Elimination Kit has been validated with most commonly used cell lines including mouse and human embryonic stem cells, iPS cells, HEK293, Hela, HepG2, HCT116, COS-7, Vero, Huh-7, MDCK, PANC-1, SW620 and U2OS.

MycoGenie Mycoplasma Elimination Kit - Kit Contents

Components MORV0012-100 MORV0012-500

MycoGenie MycoPlasma Elimination Kit

100 µl

5 x 100 µl

Storage

Store at -20℃

MycoGenie Mycoplasma Elimination Kit Protocol

Step Description

1.

Create a working solution by diluting the MycoGenie MycoPlasma Elimination reagent with fresh cell culture medium with a ratio of 1:1000.

Note:
The diluted solution should be fleshly prepared and should not be stored.

2.

Discard the contaminated cell culture media and add the fresh media containing the MycoGenie MycoPlasma Elimination solution prepared in Step 1.

Note:
During treatment, for each media replacement step, use fresh cell culture media containing MycoGenie Mycoplasma Elimination reagent.

Note:
Collect the cell culture supernatant when replacing cell culture media and use MycoGenie Rapid Mycoplasma Detection Kit (Assay Genie Cat. MORV0011) to detect mycoplasma contamination.


3.

Successful removal of mycoplasma can usually be obtained within 3 days.

Note:

A 3-day treatment means replacing the cell culture media every day for 3 days.
Note:
Adjust treatment duration according to the extent of contamination.

4.

Use the MycoGenie Rapid Mycoplasma Detection Kit (Assay Genie Cat. MORV0011) to confirm that the mycoplasma has been completely removed after treatment.

5.

After mycoplasma removal is confirmed, replace with fresh cell culture media without the MycoGenie MycoPlasma Elimination solution. After mycoplasma removal is confirmed, replace with fresh culture medium without MycoGenie MycoPlasma Elimination Kit.

Troubleshooting

1. How to treat the cells with severe mycoplasma contamination?
For severely contaminated cultures, it is recommended to extend the treatment to 7 days or even longer.

2. Is the MycoGenie MycoPlasma Elimination reagent toxic to cells?
During validation, it has been shown that the MycoGenie MycoPlasma Elimination Kit is non-toxic to most cell types. However, it is recommended to increase the dilution factor from 1:1000 to 1:2000 or even higher if cell growth is reduced during treatment.

3. Can MycoGenie MycoPlasma Elimination Kit be used with primary cell cultures?
Yes. Compared with similar products from other resources, the MycoGenie MycoPlasma Elimination Kit demonstrates is higher efficiency in eliminating mycoplasma from MEF (mouse embryo fibroblast) and other primary cell cultures, which are at high risk of mycoplasma contamination.

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