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    Phosphatases and PTP1B – Mini review(Post)

    IntroductionThe human kinome is estimated to contain 518 genes, in comparison to the estimated 180 genes that comprise the phosphatome (Arena et al., 2005). These kinase genes represent a signif

    Western Blot Protocol & Troubleshooting Guide(Page)

    Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

    Adalimumab: Mechanism, Clinical Applications, and the Role of Biosimilars(Post)

    Biologics · TNF InhibitionAdalimumab: Mechanism, Clinical Applications and BiosimilarsAdalimumab is a fully human IgG1 monoclonal antibody that binds tumour necrosis factor alpha and prevents it engag

    Cytokine Storms: What happens when the immune system overreacts?(Post)

    Immunology · HyperinflammationCytokine Storm and Cytokine Release Syndrome: Mechanisms, Triggers and MarkersA cytokine storm is a self-amplifying inflammatory response in which cytokine release drives

    ELISA vs Western Blot: Which Technique Should You Choose?(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through

    29 PhD Thesis Writing Tips(Post)

    Explore 29 essential tips for crafting a compelling and well-structured PhD thesis, blending effective writing practices with time management and academic rigor for a successful dissertation journ

    Galectin Therapeutics(Post)

    Idiopathic Pulmonary Fibrosis Idiopathic Pulmonary Fibrosis (IPF) is a chronic, irreversible and ultimately fatal disease characterized by prog

    C reactive protein(Post)

    Biomarkers · InflammationC-Reactive Protein (CRP): Structure, Function and Clinical UseCRP is the most widely measured marker of inflammation in medicine — fast to rise, fast to fall, and entirely non

    Sandwich ELISA: Step-by-Step Protocol & Troubleshooting(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

    Hemocytometer Cell Counting Procedure(Post)

    Cell Culture · ProtocolsHaemocytometer Cell Counting: Procedure, Calculation and Common ErrorsA haemocytometer turns a known chamber volume into a cell concentration, and the whole method rests on fou

    Mononuclear Phagocytes in Health and Disease(Post)

    Mononuclear phagocytes, essential in immunity and homeostasis, perform diverse roles from phagocytosis to tissue repair, influencing health and disease. Key Takeaways: Mononuclear p

    A Guide To Tau Proteins & Tauopathies(Post)

    Neuroscience · BiomarkersA Guide to Tau Proteins & TauopathiesTau keeps the neuronal cytoskeleton standing up. When its phosphorylation goes wrong, the same protein becomes the aggregate that defines

    Antibody Light Chains, Heavy Chains, Constant Regions & Tumour-Associated Antigens(Post)

    Immunology · Antibody BiologyAntibody Light Chains, Heavy Chains, Constant Regions & Tumour-Associated AntigensAn antibody’s specificity comes from its variable domains and its biological effect comes

    Typical Workflow of CRISPR-Cas9 Genome Editing(Post)

    Molecular Biology · ProtocolsTypical Workflow of CRISPR-Cas9 Genome EditingA CRISPR experiment is only as good as the steps around the cut. This guide walks the full workflow — designing and scoring a