Human IGF-II/IGF2 Recombinant Protein (RPES1602)

SKU:
RPES1602
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
€122

Description

ELISA Kit Technical Manual

Recombinant Human IGF-II/IGF2 Protein (Active)

Background:Insulin-Like Growth Factor II (IGF2) belongs to the insulin family of polypeptide growth factors that is involved in development and growth. Members of this family are structurally homologous to proinsulin, and share higher sequence identity. IGF2 is expressed only from the paternally inherited allele and believed to be secreted by the liver and to circulate in the blood. IGF2 possess growth-promoting activity and can stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF2 is influenced by placental lactogen and may play a role in fetal development.
Name:Recombinant Human IGF-II/IGF2 Protein (Active)
Size:10µg
Assay Genie SKU:RPES1602
Synonyms:Insulin-Like Growth Factor II; IGF-II; Somatomedin-A; IGF2; PP1446
Species:Human
Expression host:E.coli
Sequence:Ala25-Glu91
Accession:P01344
Mol Mass:8.9 kDa
AP Mol Mass:15 kDa
Bio Activity:Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells. The ED50 for this effect is 15.7 ng/ml.
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 5mM Hac, pH ~3.0.
Reconstitution:Please refer to the printed manual for detailed information.
Application:Cell Culture
NCBI Summary:This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
UniProt Code:P01344
NCBI GenInfo Identifier:124255
NCBI Gene ID:3481
NCBI Accession:P01344.1
UniProt Related Accession:P01344
Molecular Weight:11kDa
NCBI Full Name:Insulin-like growth factor II
NCBI Synonym Full Names:insulin like growth factor 2
NCBI Official Symbol:IGF2  
NCBI Official Synonym Symbols:GRDF; IGF-II; PP9974; C11orf43  
NCBI Protein Information:insulin-like growth factor II
UniProt Protein Name:Insulin-like growth factor II
UniProt Synonym Protein Names:Somatomedin-ACleaved into the following 3 chains:Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin
Protein Family:Insulin-like growth factor
UniProt Gene Name:IGF2  
UniProt Entry Name:IGF2_HUMAN