Human LR3-IGF Recombinant Protein (MG) (RPES3738)

SKU:
RPES3738
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
€34

Description

ELISA Kit Technical Manual

Recombinant Human LR3-IGF Protein (MG)(Active)

Background:Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF expression is regulated by growth hormone. R3 IGF is an 83 amino acid analog of IGF comprising the complete human IGF sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF has been produced with the purpose of increasing biological activity. R3 IGF is significantly more potent than human IGF-I in vitro.
Name:Recombinant Human LR3-IGF Protein (MG)(Active)
Size:10µg
Assay Genie SKU:RPES3738
Synonyms:Insulin-Like Growth Factor I; IGF-I; Mechano Growth Factor; MGF; Somatomedin-C; IGF1; IBP1
Species:Human
Expression host:E.coli
Sequence:Gly49-Ala118 (Glu51Arg)
Accession:P05019
Mol Mass:9.1 kDa
AP Mol Mass:11 kDa
Bio Activity:Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells.  The ED50 for this effect is 0.2.5 ng/ml.
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 300mM HAc-NaAc, pH 6.5.
Reconstitution:Please refer to the printed manual for detailed information.
Application:Cell Culture
UniProt Protein Function:IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region

Molecular Function:integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding

Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; exocrine pancreas development; glycolate metabolic process; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of mitosis; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; insulin-like growth factor receptor signaling pathway; response to heat; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; myoblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of glycogen biosynthetic process; positive regulation of activated T cell proliferation; signal transduction; positive regulation of smooth muscle cell migration; negative regulation of cell proliferation; platelet degranulation; glial cell differentiation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; regulation of multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cell proliferation; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; Ras protein signal transduction; blood vessel remodeling; positive regulation of Ras protein signal transduction; blood coagulation; cell motility; positive regulation of DNA replication

Disease: Insulin-like Growth Factor I Deficiency

NCBI Summary:The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
UniProt Code:P05019
NCBI GenInfo Identifier:124263
NCBI Gene ID:3479
NCBI Accession:P05019.1
UniProt Secondary Accession:P05019,P01343, Q14620, B2RWM7, E9PD02,
UniProt Related Accession:P05019
Molecular Weight:17,762 Da
NCBI Full Name:Insulin-like growth factor I
NCBI Synonym Full Names:insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol:IGF1  
NCBI Official Synonym Symbols:IGFI; IGF-I; IGF1A  
NCBI Protein Information:insulin-like growth factor I; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor IA; insulin-like growth factor IB
UniProt Protein Name:Insulin-like growth factor I
UniProt Synonym Protein Names:Mechano growth factor; MGF; Somatomedin-C
Protein Family:Insulin-like growth factor
UniProt Gene Name:IGF1  
UniProt Entry Name:IGF1_HUMAN
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