Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit (SBRS1989)

Product type:
  • Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit SBRS1989
  • Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit SBRS1989
Frequently bought together:


Human Phosphotyrosine STAT4 PharmaGenie ELISA - Kit Data

Product SKU:SBRS1989
Application:This ELISA kit recognizes human VEGFR2 phosphotyrosine.
Gene ID:6775
Gene Names:STAT4
Pathway:JAK/STAT Signaling
Synonyms:Signal transducer and activator of transcription 4
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.

Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit (SBES1989) - Product Images

Product ImageA431 cells were treated or untreated with EGF. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageHeLa cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 107 cells/ml in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Biotinylated Anti-Phosphotyrosine Antibody
4.Stop Solution
5.Assay Diluent(s)
6.Positive Control Sample
7.Lysis Buffer
8.Streptavidin-Conjugated HRP
9.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human Phosphotyrosine STAT4 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1.Prepare all reagents and samples as instructed in the manual.
2.Add 100 ul of sample or positive control to each well.
3.Incubate 2.5 h at RT or O/N at 4 °C.
4.Add 100 ul of prepared primary antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared 1X HRP-Streptavidin to each well.
7.Incubate 1 h at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.