Aldolase Activity Colorimetric Assay Kit (BN00889)

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BN00889
  • Aldolase Activity Colorimetric Assay Kit (BN00889)
  • Aldolase Activity Colorimetric Assay Kit (BN00889)
  • Aldolase Activity Colorimetric Assay Kit (BN00889)
€756

Description

ELISA Kit Technical ManualMSDS

Aldolase Activity Colorimetric Assay Kit

Aldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone. There are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy & hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase. Accurate detection of aldolase activity is valuable for diagnostic and mechanistic studies. In Assay Genie's Aldolase Activity Assay, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. Detection limit: less than 0.1 mU of aldolase activity in a variety of samples.

Figure: (a) NADH Standard Curve, (b) Aldolase Activity in rat kidney & muscle & HeLa & 3T3 cells. (c) Relative Aldolase Activity in lysates prepared from rat kidney (14.2 µg), rat muscle (10.64 µg), 3T3 cells (9.97 µg), and HeLa cells (11.35 µg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00889

Size

100 assays

Detection Method

Absorbance (OD 450 nm)

Applications

  • Measurement of Aldolase activity in various tissues/cells
  • Analysis of glycolysis and gluconeogenesis pathways

Features and Benefits

  • Rapid, convenient and high-through put adaptable
  • Kit can detect aldolase activity less than 0.1 mU

Kit Components

  • Aldolase Assay Buffer
  • Aldolase Substrate (Lyophilized)
  • Aldolase Enzyme Mix (Lyophilized)
  • Aldolase Developer (Lyophilized)
  • NADH Standard (Lyophilized)
  • Aldolase Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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