Annexin V-Biotin Apoptosis Kit (BN00021)

SKU:
BN00021
Product type:
Cellular Assay
€541 - €1,027

Description

ELISA Kit Technical ManualMSDS

Annexin V-Biotin Apoptosis Kit

The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -Beta-gal, etc.

Key Information Description

Detection Method

Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy

Size Options

  • 25 Assays
  • 100 Assays
  • 400 Assays

Species Reactivity

Mammalian

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one step staining procedure in 30 minutes
  • Fast and convenient
  • Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -Beta-gal, etc.

Kit Components

  • Annexin V-Biotin
  • 1X Binding Buffer
  • Propidium Iodide (PI)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.