Antibody FITC Labeling Kit (BN01048)

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ELISA Kit Technical ManualMSDS

Antibody FITC Labeling Kit

Assay Genie's EZLabel Antibody-FITC Labeling Kit provides an easy way to label antibodies with Fluorescein Isothiocyanate (FITC) in a user-friendly spin column format. FITC is an ideal dye for fluorescent labeling of antibodies among the most commonly used fluorescent dyes for labeling antibodies. Each spin column provided in the kit can be used to label up to 1 mg of the target antibody. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of antibody per reaction. FITC-labeled antibody has an excitation and emission wavelengths at 494 nm and 520 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis, etc.

Figure: SEC chromatogram of an Anti-BSA IgG labeled with FITC using a Superdex 200 HR 10/30 column at 0.5 ml/min. in 50 mM Tris and 0.25 M NaCl pH 7.5. The absorbance was monitored at 280 nm (Blue line) and 494 nm (Red line). The overlapping peaks of 280 nm and 494 nm indicate successful labeling of the antibody with FITC. In addition, the spin column format ensured that the purification of antibody was fast and there was no unreacted FITC left after the antibody was purified according to the kit protocol.

Key Information Description

Product SKU



For 5 x 1 mg of antibody

Detection Method

Fluorescence (Ex/Em = 494/520 nm)


  • Labeled antibodies can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis.
  • Easy to use Kit to label upto 5 mg of Antibody with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

Features and Benefits

  • All necessary reagents included.
  • Fast and efficient labeling of antibodies with FITC

Kit Components

  • EZLabel FITC
  • EZLabel Spin Column
  • EZLabel Quenching Buffer
  • EZLabel Elution Buffer

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.