ARPC4 Antibody (PACO56318)
- SKU:
- PACO56318
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | ARPC4 Antibody (PACO56318) |
| Antibody SKU: | PACO56318 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Actin-related protein 2/3 complex subunit 4 protein (2-168AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56318 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO56318 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. |
| Synonyms: | Actin-related protein 2/3 complex subunit 4 (Arp2/3 complex 20 kDa subunit) (p20-ARC), ARPC4, ARC20 |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010] |
| UniProt Code: | P59998 |
| NCBI GenInfo Identifier: | 311771642 |
| NCBI Gene ID: | 10093 |
| NCBI Accession: | NM_005718.4 |
| UniProt Secondary Accession: | P59998 |
| UniProt Related Accession: | P59998 |
| Molecular Weight: | |
| NCBI Full Name: | Homo sapiens actin related protein 2/3 complex subunit 4 (ARPC4), transcript variant 1, mRNA |
| NCBI Synonym Full Names: | actin related protein 2/3 complex subunit 4 |
| NCBI Official Symbol: | ARPC4Â Â |
| NCBI Official Synonym Symbols: | ARC20; P20-ARCÂ Â |
| NCBI Protein Information: | actin-related protein 2/3 complex subunit 4 |
| UniProt Protein Name: | Actin-related protein 2/3 complex subunit 4 |
| UniProt Synonym Protein Names: | Arp2/3 complex 20 kDa subunit |
| Protein Family: | Actin-related protein 2/3 complex |
| UniProt Gene Name: | ARPC4Â Â |
| UniProt Entry Name: | ARPC4_HUMAN |
| Antibodies |
| ARPC4 Antibody (PACO07862) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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