BCA Protein Assay Kit (BN01031)

SKU:
BN01031
  • Molecular Biology Tools BCA Protein Assay Kit BN01031
  • Molecular Biology Tools BCA Protein Assay Kit BN01031
€476

Description

ELISA Kit Technical ManualMSDS

BCA Protein Assay Kit

Assay Genie's BCA Protein Assay kit provides an ultra-sensitive method to detect and quantify total protein concentration even in the presence of detergents. The Kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 /ml). The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. The Cu+1-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 /ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. The Kit includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.

Figure: (a)Typical absorbance plots obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 h) and (b) test tube procedure (60°C for 1 h)

Key Information Description

Detection Method

Absorbance (562 nm)

Size Options

  • 2500 Assays
  • 5000 Assays

Species Reactivity

N/A

Applications

N/A

Features and Benefits

optimized for determining total protein content in low protein concentration samples (0.5-40 /ml)

Kit Components

  • BCA Reagent A
  • BCA Reagent B
  • BCA Reagent C
  • BSA (2 mg/ml)

Storage Conditions

RT

Shipping Conditions

RT

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Molecular Tools
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