Caspase-1 Colorimetric Assay Kit (BN00028)

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BN00028
€290

Description

ELISA Kit Technical ManualMSDS

Caspase-1 Colorimetric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.

Key Information Description

Detection Method

Absorbance (400 or 405 nm)

Size Options
  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays

Species Reactivity

Mammalian

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
  • Simple one-step procedure; takes 1-2 hours
  • Fast and convenient
  • Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.

Kit Components
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • YVAD-pNA (4 mM)
  • DTT (1 M)
  • Dilution Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.
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