Description
Caspase-1 Colorimetric Assay Kit
Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.
Key Information | Description |
Detection Method | Absorbance (400 or 405 nm) |
Size Options |
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Species Reactivity | Mammalian |
Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
Features and Benefits |
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Kit Components |
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Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |